Int Neurourol J.  2016 Sep;20(3):182-187. 10.5213/inj.1632718.359.

Controls of Nuclear Factor-Kappa B Signaling Activity by 5’-AMP-Activated Protein Kinase Activation With Examples in Human Bladder Cancer Cells

• Choi BH
• Lee DH
• Kim J
• Kang JH
• Park CS

• KMID: 2365710
• DOI: http://doi.org/10.5213/inj.1632718.359

Abstract

Generally, both lipopolysaccharide (LPS)- and hypoxia-induced nuclear factor kappa B (NF-κB) effects are alleviated through differential posttranslational modification of NF-κB phosphorylation after pretreatment with 5'-AMP-activated protein kinase (AMPK) activators such as 5'-aminoimidazole-4-carboxamide ribonucleotide (AICAR) or the hypoglycemic agent metformin. We found that AICAR or metformin acts as a regulator of LPS/NF-κB-or hypoxia/NF-κB-mediated cyclooxygenase induction by an AMPK-dependent mechanism with interactions between p65-NF-κB phosphorylation and acetylation, including in a human bladder cancer cell line (T24). In summary, we highlighted the regulatory interactions of AMPK activity on NF-κB induction, particularly in posttranslational phosphorylation and acetylation of NF-κB under inflammatory conditions or hypoxia environment.

Keyword

NF-kappa B; AMP-Activated Protein Kinases; Lipopolysaccharides; Hypoxia

MeSH Terms

Acetylation
AMP-Activated Protein Kinases
Anoxia
Cell Line
Humans*
Lipopolysaccharides
Metformin
NF-kappa B
Phosphorylation
Prostaglandin-Endoperoxide Synthases
Protein Kinases*
Protein Processing, Post-Translational
Urinary Bladder Neoplasms*
Urinary Bladder*
AMP-Activated Protein Kinases
Lipopolysaccharides
Metformin
NF-kappa B
Prostaglandin-Endoperoxide Synthases
Protein Kinases