Mycobiology.  2010 Jun;38(2):144-148.

Potentiation of Innate Immunity by beta-Glucans

• Seong SK
• Kim HW

Abstract

beta-Glucans have been known to exhibit antitumor activities by potentiating host immunity by an unknown mechanism. The C-type lectin dectin-1, a beta-glucan receptor, is found on the macrophage and can recognize various beta-glucans. Previously, we demonstrated the presence of beta-glucan receptor, dectin-1, on the Raw 264.7 cells as well as on murine mucosal organs, such as the thymus, the lung, and the spleen. In order to investigate immunopotentiation of innate immunity by beta-glucan, we stimulated a murine macrophage Raw 264.7 cell line with beta-glucans from Pleurotus ostreatus, Saccharomyces cerevisiae, and Laminaria digitata. Then, we analyzed cytokines such as tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 by reverse transcription-polymerase chain reaction (RT-PCR). In addition we analyzed gene expression patterns in beta-glucan-treated Raw 264.7 cells by applying total mRNA to cDNA microarray to investigate the expression of 7,000 known genes. When stimulated with beta-glucans, the macrophage cells increased TNF-alpha expression. When co-stimulation of the cells with beta-glucan and lipopolysaccharide (LPS), a synergy effect was observed by increased TNF-alpha expression. In IL-6 expression, any of the beta-glucans tested could not induce IL-6 expression by itself. However, when co-stimulation occurred with beta-glucan and LPS, the cells showed strong synergistic effects by increased IL-6 expression. Chip analysis showed that beta-glucan of P. ostreatus increased gene expressions of immunomodulating gene families such as kinases, lectin associated genes and TNF-related genes in the macrophage cell line. Induction of TNF receptor expression by FACS analysis was synergized only when co-stimulated with beta-glucan and LPS, not with beta-glucan alone. From these data, beta-glucan increased expressions of immunomodulating genes and showed synergistic effect with LPS.

Keyword

beta-Glucan; Cytokines; Laminarin; Microarray; Pleurotus ostreatus

MeSH Terms

beta-Glucans
Cell Line
Cytokines
Gene Expression
Humans
Immunity, Innate
Interleukin-6
Interleukins
Laminaria
Lectins, C-Type
Lung
Macrophages
Oligonucleotide Array Sequence Analysis
Phosphotransferases
Pleurotus
Polysaccharides
Receptors, Immunologic
Receptors, Tumor Necrosis Factor
RNA, Messenger
Saccharomyces cerevisiae
Spleen
Thymus Gland
Tumor Necrosis Factor-alpha
Cytokines
Interleukin-6
Interleukins
Lectins, C-Type
Phosphotransferases
Polysaccharides
RNA, Messenger
Receptors, Immunologic
Receptors, Tumor Necrosis Factor
Tumor Necrosis Factor-alpha
beta-Glucans