Anat Cell Biol.  2011 Sep;44(3):194-203. 10.5115/acb.2011.44.3.194.

Resveratrol activates AMPK and suppresses LPS-induced NF-kappaB-dependent COX-2 activation in RAW 264.7 macrophage cells

Affiliations
  • 1Department of Anatomy and Neurobiology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Korea. anaroh@gnu.ac.kr
  • 2Department of Pharmacology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Korea.
  • 3Department of Thoracic and Cardiovascular Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Korea.

Abstract

AMP-activated protein kinase (AMPK), an enzyme involved in energy homeostasis, regulates inflammatory responses, but its precise mechanisms are not fully understood. Recent evidence has shown that resveratrol (RES), an AMPK activator, reduces prostaglandin E2 production in lipopolysaccharide (LPS)-treated microglia. Here, we examined the effect of RES on nuclear factor kappa B (NF-kappaB) dependent cyclooxygenase (COX)-2 activation in LPS-treated RWA 264.7 macrophages. We found that treatment with RES increased AMPK activation. AMPK and acetyl CoA carboxylase phosphorylation were attenuated in cells treated with LPS+RES, compared to cells treated with LPS alone. RES inhibited tumor necrosis factor (TNF)-alpha and TNF receptor 1 in LPS-treated cells. Finally, RES inhibited LPS-induced NF-kappaB translocation into the nucleus and COX-2 expression. Moreover, the effects of 5-aminoimidazole-4-carboxamide ribose and compound C were consistent with the effects of RES in LPS-treated cells. Taken together, these results suggest that the anti-inflammatory action of RES in RAW 264.7 macrophages is dependent on AMPK activation and is associated with inhibition of the LPS-stimulated NF-kappaB-dependent COX-2 signaling pathway.

Keyword

Resveratrol; AMP-activated protein kinases; Macrophages

MeSH Terms

Acetyl-CoA Carboxylase
AMP-Activated Protein Kinases
Dinoprostone
Homeostasis
Macrophages
Microglia
NF-kappa B
Phosphorylation
Prostaglandin-Endoperoxide Synthases
Receptors, Tumor Necrosis Factor
Ribose
Stilbenes
Tumor Necrosis Factor-alpha
AMP-Activated Protein Kinases
Acetyl-CoA Carboxylase
Dinoprostone
NF-kappa B
Prostaglandin-Endoperoxide Synthases
Receptors, Tumor Necrosis Factor
Ribose
Stilbenes
Tumor Necrosis Factor-alpha
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