Biomol Ther.  2019 Jul;27(4):363-372. 10.4062/biomolther.2018.211.

7,8,4′-Trihydroxyisoflavone, a Metabolized Product of Daidzein, Attenuates 6-Hydroxydopamine-Induced Neurotoxicity in SH-SY5Y Cells

• Ko YH
• Kim SK
• Kwon SH
• Seo JY
• Lee BR
• Kim YJ
• Hur KH
• Kim SY
• Lee SY
• Jang CG

• KMID: 2455091
• DOI: http://doi.org/10.4062/biomolther.2018.211

Abstract

Daidzein isolated from soybean (Glycine max) has been widely studied for its antioxidant and anti-inflammatory activities. However, the protective effects of 7,8,4"²-trihydroxyisoflavone (THIF), a major metabolite of daidzein, on 6-hydroxydopamine (OHDA)-induced neurotoxicity are not well understood. In the current study, 7,8,4"²-THIF significantly inhibited neuronal cell death and lactate dehydrogenase (LDH) release induced by 6-OHDA in SH-SY5Y cells, which were used as an in vitro model of Parkinson's disease (PD). Moreover, pretreatment with 7,8,4"²-THIF significantly increased the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) and decreased malondialdehyde (MDA) activity in 6-OHDA-induced SH-SY5Y cells. In addition, 7,8,4"²-THIF significantly recovered 6-OHDA-induced cleaved caspase-3, cleaved caspase-9, cleaved poly-ADP-ribose polymerase (PARP), increased Bax, and decreased Bcl-2 levels. Additionally, 7,8,4"²-THIF significantly restored the expression levels of phosphorylated c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase 1/2 (ERK 1/2), phosphatidylinositol 3-kinases (PI3K)/Akt, and glycogen synthase kinase-3 beta (GSK-3β) in 6-OHDA-induced SH-SY5Y cells. Further, 7,8,4"²-THIF significantly increased the reduced tyrosine hydroxylase (TH) level induced by 6-OHDA in SH-SY5Y cells. Collectively, these results suggest that 7,8,4"²-THIF protects against 6-OHDA-induced neuronal cell death in cellular PD models. Also, these effects are mediated partly by inhibiting activation of the MAPK and PI3K/Akt/GSK-3β pathways.

Keyword

7,8,4′-Trihydroxyisoflavone; 6-Hydroxydopamine; Neurotoxicity; Apoptosis; Parkinson's disease

MeSH Terms

Apoptosis
Caspase 3
Caspase 9
Catalase
Cell Death
Glutathione
Glycogen Synthase
In Vitro Techniques
JNK Mitogen-Activated Protein Kinases
L-Lactate Dehydrogenase
Malondialdehyde
Neurons
Oxidopamine
Parkinson Disease
Phosphatidylinositol 3-Kinases
Phosphotransferases
Protein Kinases
Soybeans
Superoxide Dismutase
Tyrosine 3-Monooxygenase
Caspase 3
Caspase 9
Catalase
Glutathione
Glycogen Synthase
JNK Mitogen-Activated Protein Kinases
L-Lactate Dehydrogenase
Malondialdehyde
Oxidopamine
Phosphatidylinositol 3-Kinases
Phosphotransferases
Protein Kinases
Superoxide Dismutase
Tyrosine 3-Monooxygenase