Korean J Vet Res.  2014 Mar;54(1):1-6.

Neuroprotective effects of resveratrol on 6-hydroxydopamine-induced damage of SH-SY5Y cell line

Affiliations
  • 1College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea. mbwie@kangwon.ac.kr
  • 2Neuropsychopharmacology and Toxicology Program, College of Pharmacy, Kangwon National University, Chuncheon 200-701, Korea.

Abstract

Parkinson's disease is known to exhibit progressive degeneration of the dopaminergic neurons in the substantia nigra via inhibition of glutathione metabolism. It is well known that 6-Hydroxydopamine (6-OHDA) induces Parkinson's disease-like symptoms, while resveratrol (3,5,4'-trihydroxystilbene) has been shown to have anti-inflammatory and antioxidant effects. In the present study, we investigated the neuroprotective effects of resveratrol, a phytoalexin found in grapes and various plants, on 6-OHDA-induced cell damage to the SH-SY5Y human neuroblastoma cell line. Resveratrol (5 and 10 microM) inhibited 6-OHDA (60 microM)-induced cytotoxicity in SH-SY5Y cells and induced a reduction of the number of apoptotic nuclei caused by 6-OHDA treatment. Additionally, the total apoptotic rate of cells treated with both resveratrol (10 microM) and 6-OHDA (60 microM) was less than that of 6-OHDA treated cells. Resveratrol also dose-dependently (1, 5 and 10 microM) scavenged reactive oxygen species (ROS) induced by 6-OHDA in SH-SY5Y cells and prevented depletion of glutathione in response to the 6-OHDA-induced cytotoxicity in the glutathione assay. Overall, these results indicate that resveratrol exerts a neuroprotective effect against 6-OHDA-induced cytotoxicity of SH-SY5Y cells by scavenging ROS and preserving glutathione.

Keyword

6-hydroxydopamine; apoptosis; Parkinson's disease; resveratrol; SH-SY5Y cell line

MeSH Terms

Antioxidants
Apoptosis
Cell Line*
Dopaminergic Neurons
Glutathione
Humans
Metabolism
Neuroblastoma
Neuroprotective Agents*
Oxidopamine
Parkinson Disease
Reactive Oxygen Species
Substantia Nigra
Vitis
Antioxidants
Glutathione
Neuroprotective Agents
Oxidopamine
Reactive Oxygen Species
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