Int Neurourol J.  2019 Nov;23(Suppl 2):S111-S118. 10.5213/inj.1938190.095.

Neuregulin-1 Protects Neuronal Cells Against Damage due to CoCl2-Induced Hypoxia by Suppressing Hypoxia-Inducible Factor-1α and P53 in SH-SY5Y Cells

• Yoo SY
• Yoo JY
• Kim HB
• Baik TK
• Lee JH
• Woo RS

• KMID: 2467319
• DOI: http://doi.org/10.5213/inj.1938190.095

Abstract

PURPOSE
Hypoxia-mediated neurotoxicity contributes to various neurodegenerative disorders, including Alzheimer disease. Neuregulin-1 (NRG1) plays an important role in the development and plasticity of the brain. The aim of the present study was to investigate the neuroprotective effect and the regulating hypoxic inducible factor of NRG1 in cobalt chloride (CoClâ‚‚) induced hypoxia.
METHODS
Hypoxia was induced in SH-SY5Y cells by CoClâ‚‚ treatment. SH-SY5Y cells were pretreated with NRG1 and then treated with CoClâ‚‚. Western blotting, immunocytochemistry, and lactate dehydrogenase (LDH) release assays were performed to examine neuroprotective properties of NRG1 in SH-SY5Y cells.
RESULTS
Our data showed that CoCl₂ induced cytotoxicity and changes of hypoxia-inducible factor-1α (HIF-1α) and p53 expression in SH-SY5Y cells. However, pretreatment with NRG1 inhibited CoCl₂-induced accumulation of HIF-1α and p53 stability. In addition, NRG1 significantly attenuated cell death of SH-SY5Y induced by CoCl₂.
CONCLUSIONS
NRG1 can regulate HIF-1α and p53 to protect neurons against hypoxic damage.

Keyword

Neuregulin-1; Hypoxia; CoCl₂; HIF-1α; p53

MeSH Terms

Alzheimer Disease
Anoxia*
Blotting, Western
Brain
Cell Death
Cobalt
Immunohistochemistry
L-Lactate Dehydrogenase
Neuregulin-1*
Neurodegenerative Diseases
Neurons*
Neuroprotective Agents
Plastics
Cobalt
L-Lactate Dehydrogenase
Neuregulin-1
Neuroprotective Agents
Plastics