Korean J Pediatr.  2008 Oct;51(10):1102-1111. 10.3345/kjp.2008.51.10.1102.

Neuroprotective effects of resveratrol via anti-apoptosis on hypoxic-ischemic brain injury in neonatal rats

Affiliations
  • 1Department of Pediatrics, School of Medicine, Catholic University of Daegu, Daegu, Korea. wootykim@cu.ac.kr
  • 2Department of Ophthalmology, Dongguk University College of Medicine, Gyeongju, Korea.
  • 3Department of Pediatrics, Masan Samsung Hospital, School of Medicine, Sungkyunkwan University, Masan, Korea.

Abstract

PURPOSE: Resveratrol, extracted from red wine and grapes, has an anti-cancer effect, an antiinflammatory effect, and an antioxidative effect mainly in heart disease and also has neuroprotective effects in the adult animal model. No studies for neuroprotective effects during the neonatal periods have been reported. Therefore, we studied the neuroprotective effect of resveratrol on hypoxic-ischemic brain damage in neonatal rats via anti-apoptosis.
METHODS
Embryonic cortical neuronal cell culture of rat brain was performed using pregnant Sprague-Dawley (SD) rats at 18 days of gestation (E1 8) for the in vitro approach. We injured the cells with hypoxia and administered resveratrol (1, 10, and 30 microg/mL) to the cells at 30 minutes before hypoxic insults. In addition, unilateral carotid artery ligation with hypoxia was induced in 7 -day-old neonatal rats for the in vivo approach. We injected resveratrol (30 mg/kg) intraperitoneally into animal models. Real-time PCR and Western blotting were performed to identify the neuroprotective effects of resveratrol through anti-apoptosis.
RESULTS
In the in vitro approach of hypoxia, the expression of Bax, caspase-3, and the ratio of Bax/Bcl-2, indicators of the level of apoptosis, were significantly increased in the hypoxia group compared to the normoxia group. In the case of the resveratrol-treated group, expression was significantly decreased compared to the hypoxia group. And the results in the in vivo approach were the same as in the in vitro approach.
CONCLUSION
The present study demonstrates that resveratrol plays neuroprotective role in hypoxic-ischemic brain damage during neonatal periods through the mechanism of anti-apoptosis.

Keyword

Resveratrol; Hypoxic-ischemic brain injury; Neuroprotective; Newborn; Apoptosis

MeSH Terms

Adult
Animals
Anoxia
Apoptosis
Blotting, Western
Brain
Brain Injuries
Carotid Arteries
Caspase 3
Cell Culture Techniques
Heart Diseases
Humans
Infant, Newborn
Ligation
Models, Animal
Neurons
Neuroprotective Agents
Pregnancy
Rats
Real-Time Polymerase Chain Reaction
Stilbenes
Vitis
Wine
Caspase 3
Neuroprotective Agents
Stilbenes
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