Clin Exp Otorhinolaryngol.  2019 May;12(2):196-205. 10.21053/ceo.2019.00094.

Antiallergic Effect of Hizikia fusiformis in an Ovalbumin-Induced Allergic Rhinitis Mouse Model

Affiliations
  • 1Center of Morphological Experiment, Medical College of Yanbian University, Yanji, China.
  • 2Department of Biochemical and Polymer Engineering, Chosun University, Gwangju, Korea.
  • 3Department of Otorhinolaryngology-Head and Neck Surgery, Chosun University College of Medicine, Gwangju, Korea. jeviolin@hanmail.net

Abstract


OBJECTIVES
The extract of Hizikia fusiformis is known to exhibit anticancer, antiatopic and antioxidant activities. We aimed to investigate the extract of H. fusiformis on allergic rhinitis inflammation in a mouse model.
METHODS
The 4-week-old BALB/c mice were randomly assigned into four groups: group A, control group (n=9); group B, allergic rhinitis group (n=10); group C (n=10) received 300 mg/kg of H. fusiformis during nasal challenging period; group D (n=10) received 600 mg/kg of H. fusiformis during general sensitization period and 300 mg/kg of H. fusiformis during nasal challenging period. Allergic inflammation was made with ovalbumin (OVA) and alum then challenged intranasally with OVA. H. fusiformis was intraperitoneally administered 3 hours before the OVA administration. Allergic symptom score and the levels of immunoglobulin G1 (IgG1), IgG2a, OVA-specific IgE antibodies, levels of cytokines in the nasal mucosa and in spleen cell culture supernatant, such as tumor necrosis factor alpha (TNF-α), interleukin 4 (IL-4), IL-5, IL-13, and IL-10 were assessed. The percentage of regulatory T cell was analyzed by flow cytometry. Eosinophilic infiltration and goblet cell hyperplasia were also evaluated.
RESULTS
H. fusiformis administered groups C and D showed significant inhibitory effects on nasal symptoms, IL-13 mRNA expression and eosinophil infiltration/goblet cell hyperplasia in the nasal tissue; OVA-specific IgE production in serum (P<0.05). In group D, H. fusiformis treatment downregulated IL-4, IL-5, IL-13, TNF-α, and IL-10 cytokine expression in splenocyte culture as well as significantly decreased IgG2a, IgG1 levels in serum compared with group B (P<0.05). However, the expressions of IL-5, interferon-γ and forkhead box P3 mRNA did not change in groups C and D.
CONCLUSION
H. fusiformis could induce antiallergic inflammation by suppressing the T-helper type 2 cytokine production (IL-13) locally and systemically, OVA-specific IgE formation, goblet cell hyperplasia, and eosinophilic infiltration in a mouse model of allergic rhinitis. Thus, H. fusiformis could be considered as a potential therapeutic agent in treating allergic rhinitis.

Keyword

Allergic Rhinitis; Th2 Cells; Inflammation; Mice

MeSH Terms

Animals
Antibodies
Cell Culture Techniques
Cytokines
Eosinophils
Flow Cytometry
Goblet Cells
Hyperplasia
Immunoglobulin E
Immunoglobulin G
Immunoglobulins
Inflammation
Interleukin-10
Interleukin-13
Interleukin-4
Interleukin-5
Mice*
Nasal Mucosa
Ovalbumin
Ovum
Rhinitis, Allergic*
RNA, Messenger
Spleen
Th2 Cells
Tumor Necrosis Factor-alpha
Antibodies
Cytokines
Immunoglobulin E
Immunoglobulin G
Immunoglobulins
Interleukin-10
Interleukin-13
Interleukin-4
Interleukin-5
Ovalbumin
RNA, Messenger
Tumor Necrosis Factor-alpha
Full Text Links
  • CEO
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error