Biomol Ther.  2018 Nov;26(6):584-590. 10.4062/biomolther.2018.049.

Cytoprotective Effect of Taurine against Hydrogen Peroxide-Induced Oxidative Stress in UMR-106 Cells through the Wnt/β-Catenin Signaling Pathway

• Lou J
• Han D
• Yu H
• Yu G
• Jin M
• Kim SJ

• KMID: 2427512
• DOI: http://doi.org/10.4062/biomolther.2018.049

Abstract

Osteoporosis development is closely associated with oxidative stress and reactive oxygen species (ROS). Taurine has potential antioxidant effects, but its role in osteoblasts is not clearly understood. The aim of this study was to determine the protective effects and mechanisms of actions of taurine on hydrogen peroxide (H₂O₂)-induced oxidative stress in osteoblast cells. UMR-106 cells were treated with taurine prior to H₂O₂ exposure. After treatment, cell viability, apoptosis, intracellular ROS production, malondialdehyde content, and alkaline phosphate (ALP) activity were measured. We also investigated the protein levels of β-catenin, ERK, CHOP and NF-E2-related factor 2 (Nrf2) along with the mRNA levels of Nrf2 downstream antioxidants. The results showed that pretreatment of taurine could reverse the inhibition of cell viability and suppress the induced apoptosis in a dose-dependent manner: taurine significantly reduced H₂O₂-induced oxidative damage and expression of CHOP, while it induced protein expression of Nrf2 and β-catenin and activated ERK phosphorylation. DKK1, a Wnt/β-catenin signaling inhibitor, significantly suppressed the taurine-induced Nrf2 signaling pathway and increased CHOP. Activation of ERK signaling mediated by taurine in the presence of H₂O₂ was significantly inhibited by DKK1. These data demonstrated that taurine protects osteoblast cells against oxidative damage via Wnt/β-catenin-mediated activation of the ERK signaling pathway.

Keyword

Taurine; Oxidative stress; Antioxidants; Wnt/β-catenin; Osteoblast

MeSH Terms

Antioxidants
Apoptosis
Cell Survival
Hydrogen Peroxide
Hydrogen*
Malondialdehyde
NF-E2-Related Factor 2
Osteoblasts
Osteoporosis
Oxidative Stress*
Phosphorylation
Reactive Oxygen Species
RNA, Messenger
Taurine*
Antioxidants
Hydrogen
Hydrogen Peroxide
Malondialdehyde
NF-E2-Related Factor 2
RNA, Messenger
Reactive Oxygen Species
Taurine