J Korean Ophthalmol Soc.  2008 Oct;49(10):1665-1670.

Effect of Hydrogen Peroxide-induced Oxidative Stress on the Senescence of Trabecular Meshwork Cells

• Kim JW
• Kim SH
• Lee JH

Abstract

PURPOSE
To investigate the effects of oxidative stress on the senescence of trabecular meshwork (TM) cells and the effect of L-ascorbic acid (LAA) against oxidative stress-induced senescence.
METHODS
Primary cultured human TM cells were exposed to 0.05 or 0.1 mM hydrogen peroxide for 30 minutes and incubated for 1 week with or without co-exposure of LAA. Cellular survival, nitrite production, and senescence were assessed with MTT, Griess, and SA-beta-gal assays, respectively.
RESULTS
Hydrogen peroxide decreased cellular survival and NO production accompanied increased cellular senescence. LAA did not prevent hydrogen peroxide-induced senescence.
CONCLUSIONS
Oxidative stress-induced senescence of TM cells may be related to the dysfunction of trabecular meshwork in glaucoma.

Keyword

Ascorbic acid; Hydrogen peroxide; Oxidative stress; Senescence; Trabecular meshwork cells

MeSH Terms

Aging
Ascorbic Acid
Cell Aging
Glaucoma
Humans
Hydrogen
Hydrogen Peroxide
Oxidative Stress
Trabecular Meshwork
Ascorbic Acid
Hydrogen
Hydrogen Peroxide

Figure

• 

  Figure 1. Effect of hydrogen-peroxide-induced oxidative stress on the production of NO in cultured trabecular meshwork cells. Hydrogen peroxide inhibited NO production significnatly, which was abolished by co-exposed L-ascorbic acid (LAA) (* p<0.05).

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  Figure 2. Effect of hydrogen peroxide on the survival of trabecular meshwork cells. Hydrogen peroxide decreased cellular survival significantly in a dose dependent manner. Co-exposed 0.1 mM L-ascorbic acid did not affect on the survival (* p<0.05).

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  Figure 3. Photograph of SA-β-gal positive senescent trabecular meshwork cells (blue color) after exposed to 0.05 mM of hydrogen peroxide. Original magnification ×100.

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  Figure 4. Effect of hydrogen peroxide on the induction of cellular senescence in cultured trabecular meshwork cells. Hydrogen peroxide increased cellular senescence significantly, which was not abolished by co-exposed 0.1 mM L-ascorbic acid (* p<0.05).

Reference

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