Korean J Ophthalmol.  2005 Sep;19(3):227-232. 10.3341/kjo.2005.19.3.227.

Ascorbic Acid Enhances Nitric Oxide Production in Trabecular Meshwork Cells

• Kim JW

• KMID: 754429
• DOI: http://doi.org/10.3341/kjo.2005.19.3.227

Abstract

PURPOSE
This study investigated the role of ascorbic acid on the production of nitric oxide (NO) in the trabecular meshwork (TM) cells. METHODS: After primarily cultured human TM cells were exposed to 1, 10, and 100 micrometer of L-ascorbic acid (LAA), with or without co-administration of 1 mM sodium nitroprusside or 100 micrometer hydrogen peroxide for 48 hr, cellular survival and NO production were measured with MTT and Griess assay, respectively. RESULTS: LAA significantly potentiated NO production in a dose-dependent manner (p< 0.05) without affecting cell viability. LAA increased cell viability after hydrogen peroxide-induced oxidative stress in a dose-dependent manner. LAA enhanced NO production in TM cells and showed a cytoprotective effect against hydrogen peroxide-induced oxidative stress. CONCLUSIONS: LAA might be involved in the regulation of trabecular outflow by enhancing NO production in TM cells.

Keyword

Ascorbic acid; Hydrogen peroxide; Nitric oxide; Trabecular meshwork cell

MeSH Terms

Trabecular Meshwork/cytology/*drug effects/*metabolism
Nitric Oxide/*biosynthesis
Humans
Dose-Response Relationship, Drug
Cells, Cultured
Cell Survival/drug effects
Ascorbic Acid/administration & dosage/*pharmacology

Figure

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  Fig. 1. Effect Of L-ascorbic acid on the viability of cultured trabecular meshwork cells. L—ascorbic acid does not affect significantly on the Viability of trabecular meshwork cells up to 100 uM

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  Fig. 2. Effect of 3 mM sodium nitroprusside and 1 mM hydrogen peroxide on the survival of cultured trabecular meshwork cells (∗p<0.05).

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  Fig. 3. Effect of L—ascorbic acid on the production of nitrite in cultured trabecular meshwork cells. L—ascorbic acid potentiate nitrate production significantly in a dose-dependent manner and L—NAME inhibited nitrite production significantly (∗p<0.05).

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  Fig. 4. Effect of L—ascorbic acid on the production of nitric oxide exposed to 3 mM sodium nitroprusside and 1 mM hydrogen peroxide in Cultured trabecular meshwork cells (∗p<0.05).

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