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Effects of different culture systems on the culture of prepuberal buffalo (Bubalus bubalis) spermatogonial stem cell-like cells in vitro

Li TT, Geng SS, Xu HY, Luo AL, Zhao PW, Yang H, Liang XW, Lu YQ, Yang XG, Lu KH

Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems...
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A Simple and Nonenzymatic Method to Isolate Human Corpus Cavernosum Endothelial Cells and Pericytes for the Study of Erectile Dysfunction

Yin GN, Ock J, Choi MJ, Song KM, Ghatak K, Minh NN, Kwon MH, Seong DH, Jin HR, Ryu JK, Suh JK

PURPOSE: To establish a simple and nonenzymatic technique to isolate endothelial cells (ECs) and pericytes from human corpus cavernosum tissue and to evaluate the angiogenic ability of the human cavernous...
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Exosomes as Therapeutic Vehicles for Cancer

Lim W, Kim HS

BACKGROUND: Exosomes are membrane-enclosed extracellular vesicles implicated in cell-cell communication. Exosomes contain proteins, mRNAs, non-coding RNAs (miRNAs and lncRNAs) and lipids that are derived from producing cells. These nano-sized vesicles...
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Effects of three-dimensionally printed polycaprolactone/β-tricalcium phosphate scaffold on osteogenic differentiation of adipose tissue- and bone marrow-derived stem cells

Park H, Kim JS, Oh EJ, Kim TJ, Kim HM, Shim JH, Yoon WS, Huh JB, Moon SH, Kang SS, Chung HY

BACKGROUND: Autogenous bone grafts have several limitations including donor-site problems and insufficient bone volume. To address these limitations, research on bone regeneration is being conducted actively. In this study, we...
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Xenotransplantation of Cultured Human Corneal Endothelial Cell Sheets

Lee YP, Chung TY, Hyon JY, Shin YJ

PURPOSE: To investigate the possibility of transplantation into rabbits of collagenase-induced cultured human corneal endothelial cell plate-rabbit corneal stromal complexes. METHODS: Human corneal endothelial cells were isolated from residual corneal limbus...
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Long Non-Coding RNA TUG1 Promotes Proliferation and Inhibits Apoptosis of Osteosarcoma Cells by Sponging miR-132-3p and Upregulating SOX4 Expression

Li G, Liu K, Du X

PURPOSE: Long non-coding RNA taurine upregulated gene 1 (TUG1) is reported to be a vital regulator of the progression of various cancers. This study aimed to explore the exact roles...
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Functional Recovery Following the Transplantation of Olfactory Ensheathing Cells in Rat Spinal Cord Injury Model

Muniswami , Tharion G

STUDY DESIGN: Olfactory ensheathing cells (OECs) from rat olfactory mucosa were cultured, characterized, and transplanted into a rat model of spinal cord injury (SCI). PURPOSE: To evaluate different doses of OECs...
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Effects of Several Cosmetic Preservatives on ROS-Dependent Apoptosis of Rat Neural Progenitor Cells

Ryu O, Park BK, Bang M, Cho KS, Lee SH, Gonzales E, Yang SM, Kim S, Eun PH, Lee JY, Kim KB, Shin CY, Kwon KJ

Benzalkonium chloride, diazolidinyl urea, and imidazolidinyl urea are commonly used preservatives in cosmetics. Recent reports suggested that these compounds may have cellular and systemic toxicity in high concentration. In addition,...
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Gintonin, an exogenous ginseng-derived LPA receptor ligand, promotes corneal wound healing

Kim HJ, Kim JY, Lee BH, Choi SH, Rhim H, Kim HC, Ahn SY, Jeong SW, Jang M, Cho IH, Nah SY

Ginseng gintonin is an exogenous ligand of lysophosphatidic acid (LPA) receptors. Accumulating evidence shows LPA helps in rapid recovery of corneal damage. The aim of this study was to evaluate...
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Recombinant Human Erythropoietin Exerts Neuroprotective Effects via Modulation of Nitric Oxide Synthase on Hypoxic-Ischemic Brain Injury in Neonatal Rats

Lee SG, Lee EJ, Kim WT

OBJECTIVE: Erythropoietin (EPO) has neuroprotective effects in many animal models of brain injury including hypoxic-ischemic (HI) encephalopathy, trauma- and excitotoxicity. Current studies have demonstrated the neuroprotective effects of EPO, but...
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Platelet-Derived Growth Factor Receptor-Positive Pericytic Cells of White Adipose Tissue from Critical Limb Ischemia Patients Display Mesenchymal Stem Cell-Like Properties

Kim EJ, Seo SG, Shin HS, Lee DJ, Kim JH, Lee DY

BACKGROUND: The pericytes in the blood vessel wall have recently been identified to be important in regulating vascular formation, stabilization, remodeling, and function. We isolated and identified pericyte-like platelet-derived growth...
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Induction of Apoptosis in Intestinal Toxicity to a Histone Deacetylase Inhibitor in a Phase I Study with Pelvic Radiotherapy

Kalanxhi E, Risberg K, Barua IS, Dueland S, Waagene S, Andersen S, Pettersen , Lindvall J, Redalen KR, Flatmark K, Ree AH

PURPOSE: When integrating molecularly targeted compounds in radiotherapy, synergistic effects of the systemic agent and radiation may extend the limits of patient tolerance, increasing the demand for understanding the pathophysiological...
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Role of TGFBIp in Wound Healing and Mucin Expression in Corneal Epithelial Cells

Maeng YS, Lee GH, Lee B, Choi SI, Kim TI, Kim EK

PURPOSE: Transforming growth factor-β-induced protein (TGFBIp) is highly expressed in the cornea, and mutant TGFBIp induces corneal diseases. However, the function of TGFBIp in cornea epithelium is not fully investigated....
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Relaxin Modulates the Expression of MMPs and TIMPs in Fibroblasts of Patients with Carpal Tunnel Syndrome

Kang YM, Lee HM, Moon SH, Kang H, Choi YR

PURPOSE: The aim of this study was to investigate the anti-fibrotic effect of relaxin in subsynovial fibroblasts activated by transforming growth factor beta (TGF-β). MATERIALS AND METHODS: To test the anti-fibrotic...
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A Questionnaire Survey of Quality Control of Flow Cytometry in Korea and Development of New Quality-Control Materials

Shim JS, Hwang SM, Kim IS, Shin SY, Oh JY, Lee KJ, Kong SY

BACKGROUND: There are few commercial quality-control (QC) materials for internal QC of flow cytometric analysis, especially for leukemia/lymphoma immunophenotyping. The purpose of this study was to investigate the current QC...
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Generation of Induced Pluripotent Stem Cells and Neural Stem/Progenitor Cells from Newborns with Spina Bifida Aperta

Bamba Y, Nonaka M, Sasaki N, Shofuda T, Kanematsu , Suemizu H, Higuchi Y, Pooh RK, Kanemura Y, Okano H, Yamasaki M

STUDY DESIGN: We established induced pluripotent stem cells (iPSCs) and neural stem/progenitor cells (NSPCs) from three newborns with spina bifida aperta (SBa) using clinically practical methods. PURPOSE: We aimed to develop...
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11β-Hydroxysteroid Dehydrogenase Type 1 Inhibition Attenuates the Adverse Effects of Glucocorticoids on Dermal Papilla Cells

Lee SE, Lee EY, Kang SJ, Lee SH

PURPOSE: Glucocorticoids, stress-related hormones, inhibit hair growth. Intracellular glucocorticoid availability is regulated by 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1). 11β-HSD1 was recently detected in keratinocytes and fibroblasts. However, the expression of...
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Docosahexaenoic acid-mediated protein aggregates may reduce proteasome activity and delay myotube degradation during muscle atrophy in vitro

Shin SK, Kim JH, Lee JH, Son YH, Lee MW, Kim HJ, Noh SA, Kim KP, Kim IG, Lee MJ

Proteasomes are the primary degradation machinery for oxidatively damaged proteins that compose a class of misfolded protein substrates. Cellular levels of reactive oxygen species increase with age and this cellular...
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Transplantation of Mesenchymal Stem Cells for Acute Spinal Cord Injury in Rats: Comparative Study between Intralesional Injection and Scaffold Based Transplantation

Kim YC, Kim YH, Kim JW, Ha KY

Experimental stem cell therapy for spinal cord injury (SCI) has been extensively investigated. The selection of effective cell transplantation route is also an important issue. Although various types of scaffold...
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Immunologic properties of differentiated and undifferentiated mesenchymal stem cells derived from umbilical cord blood

Lee HJ, Kang KS, Kang SY, Kim HS, Park SJ, Lee SY, Kim KD, Lee HC, Park JK, Paik WY, Lee L, Yeon SC

The expression of immunogenic markers after differentiation of umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and requires extensive in vitro and in vivo testing for...
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