J Korean Med Sci.
1991 Sep;6(3):246-250. 10.3346/jkms.1991.6.3.246.
Effect of glucose on the expression of c-myc gene in cultured RINm5F cell
- Affiliations
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- 1Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.
- KMID: 1421861
- DOI: http://doi.org/10.3346/jkms.1991.6.3.246
Abstract
- The study was designed to examine the effect of glucose on the expression of c-myc gene in cultured RINm5F cells. After monolayer culture was established in RPMI 1640 media supplemented with 10% fetal calf serum (FCS), the cells were cultured in various concentrations of glucose and 1 or 10% FCS for another 24 hours. A mRNA was extracted from the cultured cells by a single step method, and Northern analysis was done to detect RNA band. A 0.5 kilobase single band was detected as c-myc mRNA. The expression of c-myc gene mRNA was reduced with increased concentration of glucose with 1% FCS. However, supplementation of 10% FCS abolished the effect of glucose on expression of c-myc gene. These findings suggested that glucose in conjunction with other growth promoting factors played an important role in expression of oncogene and cell growth in RINm5F cells.
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