Korean J Phys Anthropol.  1998 Jun;11(1):33-40.

Expression Correlation Between NM23/NDP Kinase and c-myc in NM23/NDP Kinase Transfectant Cell Lines

• Lee KY
• Song DW
• Kim DJ
• Kim DS
• Chang SI
• Lee IH

Abstract

Since Steeg, et al.(1988) identified NM23/NDP kinase as non -metastasis gene, other multiple functions of have reported. One of them, Postel, et al.(1993) suggested that transcription factor PuF, being encoded by NM23 -H2/NDP kinase gene, interacts with nuclease hypersensitive element located upstream of the c -myc gene. C -myc amplification and activation can be present in squamous cell carcinoma of the head and neck as well as in an increased metastatic propensity for individual tumor. To clarify the role of NM23/NDP kinase on c -myc expression, comparison of these two gene expressions in cell lines was done. No direct correlation of expression kinetics was found. A plasmid containing human c -myc fragment was cloned upstream of chloramphenicol acetyltransferase (CAT) gene. When murine melanoma cell line was cotransfected with a murine NM23 -M2 including expression vector and c -myc CAT, CAT activity was elevated, while no change of CAT activity was found in the cotransfectant of human NM23 -H2 and c -myc CAT. Data suggest that murine NM23 -M2 gene transactivates c -myc gene indirectly with a cellular factor in murine cell line which dose not work with human NM23 -H2 gene. Additionally, we found same kinetics of NM23 -H2/NDP kinase and c -myc expression change correlated with proliferation of PLC/PRF/5 which was induced by HGF.

Keyword

NM23/NDP Kinase; c -myc; Transactivation

MeSH Terms

Animals
Carcinoma, Squamous Cell
Cats
Cell Line*
Chloramphenicol O-Acetyltransferase
Clone Cells
Gene Expression
Head
Humans
Kinetics
Melanoma
Neck
Phosphotransferases*
Plasmids
Transcription Factors
Transcriptional Activation
Chloramphenicol O-Acetyltransferase
Phosphotransferases
Transcription Factors