J Korean Ophthalmol Soc.  2003 Feb;44(2):485-490.

Effect of Mitomycin C on the Proliferation and Nitric Oxide Production in the Cultured Trabecular Meshwork Cells

• Cho BJ
• Kim JW

Abstract

PURPOSE
To investigate the effect of mitomycin C on the proliferation of cultured trabecular cells and its relation to nitric oxide (NO) production. METHODS: The effect of NO donor, SIN-1, on the proliferation of primarily cultured porcine trabecular meshwork cells was studied with MTT assay. After treatment with mitomycin C at various concentrations for 1 hour, the proliferation was assessed by MTT assay and the production of nitrite was assessed by Griess reaction after 24 hours and 3 days respectively. RESULTS: SIN-1 significantly inhibited proliferation of cultured trabecular meshwork cells (p<0.05). Mitomycin C also suppressed the cellular proliferation(p<0.05) but did not affect the production of nitrite (p>0.05). CONCLUSIONS: Both NO donor and mitomycin C have an inhibitory effect on the proliferation of trabecular meshwork cells. However, inhibitory effect of mitomycin C was not related to the production of NO.

Keyword

Culture; Griess reaction; Mitomycin C; MTT; Nitric oxide; Proliferation; Trabecular meshwork cells

MeSH Terms

Humans
Mitomycin*
Nitric Oxide*
Tissue Donors
Trabecular Meshwork*
Mitomycin
Nitric Oxide