J Korean Ophthalmol Soc.  2003 Dec;44(12):2905-2910.

Effect of beta-adrenergics on the Survival and Production of Nitric Oxide in the Cultured Trabecular Meshwork Cells

• Jeon YH
• Kim SH
• Kim JW

Abstract

PURPOSE
To investigate the effect of beta-adrenergics on the production of nitric oxide (NO) in cultured trabecular meshwork cells. METHODS: Primarily cultured porcine trabecular meshwork cells were exposed to timolol and to propranolol at various concentrations. Cellular survival was assessed by MTT assay and the production of nitrite was assessed by Griess reaction after 24 hours and 3 days respectively. Also investigated was the effect of co-administraton of timolol and isoproterenol. RESULTS: After exposure for 24 hours, neither drug affected the cellular survival. Timolol and propranolol inhibited the production of NO significantly (p<0.05). Isoproterenol abolished timolol-induced inhibition of NO production after 24 hours. These results were similar after exposure for 3 days. CONCLUSIONS: Both timolol and propranolol inhibit the production of NO in trabecular meshwork cells and isoproterenol abolished this effect. These results suggest that beta-adrenerics involves in the production of NO in trabecular meshwork cells.

Keyword

Isoproterenol; Nitric oxide; Propranolol; Timolol; Trabecular meshwork cells

MeSH Terms

Isoproterenol
Nitric Oxide*
Propranolol
Timolol
Trabecular Meshwork*
Isoproterenol
Nitric Oxide
Propranolol
Timolol