J Korean Ophthalmol Soc.  2004 Dec;45(12):2127-2132.

Effect of Hypoxia on the Survival and Production of Nitric Oxide in Trabecular Meshwork Cells

• Jin YH
• Kim JW

Abstract

PURPOSE
To investigate the effect of hypoxia on the survival and nitric oxide (NO) production of cultured trabecular meshwork (TM) cells. METHODS: After inducing chemical hypoxia with sodium cyanide, the survival and nitrite production of the primarily cultured porcine TM cells were assessed with MTT and Griess assays. The effect of NOS inhibitor, N(omega)-Nitro-L-arginine methyl ester (L-NAME), was also assessed. Flow cytometry using annexin/PI was done to evaluate apoptosis. RESULTS: Chemical hypoxia decreased TM cell survival significantly (p<0.05) with increased NO production. This hypoxia-induced antiproliferative effect was abolished by L-NAME (p<0.05). Flow cytometric analysis revealed that hypoxia induced apoptosis of TM cells, which was inhibited by L-NAME. CONCLUSIONS: Hypoxia decreases the survival of TM cells and induced apoptosis, accompanied by increased NO production. The hypoxia-induced decreased survival of TM cells may be mediated by NO.

Keyword

Apoptosis; Hypoxia; Nitric oxide; Trabecular meshwork cells

MeSH Terms

Anoxia*
Apoptosis
Cell Survival
Flow Cytometry
NG-Nitroarginine Methyl Ester
Nitric Oxide*
Sodium Cyanide
Trabecular Meshwork*
NG-Nitroarginine Methyl Ester
Nitric Oxide
Sodium Cyanide