J Korean Ophthalmol Soc.  2011 Aug;52(8):970-974. 10.3341/jkos.2011.52.8.970.

Effect of Genistein on the Survival and Production of Nitric Oxide in Trabecular Meshwork Cells

• Hong JH
• Kim YY
• Kim JW

• KMID: 2214775
• DOI: http://doi.org/10.3341/jkos.2011.52.8.970

Abstract

PURPOSE
To investigate the effects of genistein on the survival and production of nitric oxide in cultured human trabecular meshwork cells (HTMC).
METHODS
Primarily cultured HTMC were exposed to 0, 1, and 10 microm genistein using serum-deprived media. Production of nitric oxide and eNOS activity were assessed with the Griess assay and RT-PCR after exposure to genistein for 10 min and one day. Cellular survival was assessed via MTT assay after exposure to genistein for one day.
RESULTS
Genistein significantly increased the production of nitric oxide after exposure for 1 min at 10 microm and for 1 day at 1 microm under serum-deprived conditions. Genistein increased eNOS activity and cellular survival in HTMC.
CONCLUSIONS
Genistein increases cellular survival under serum-deprived conditions, accompanied with an increase in nitric oxide production after both short-term and long-term exposures.

Keyword

Genistein; Nitric oxide; Survival; Trabecular meshwork cells

MeSH Terms

Genistein
Humans
Nitric Oxide
Trabecular Meshwork
Genistein
Nitric Oxide

Figure

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  Figure 1. Effect of genistein on the production of nitric oxide in trabecular meshwork cells after exposure for 10 minutes (* p < 0.05).

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  Figure 2. Effect of genistein on the production of nitric oxide in trabecular meshwork cells after exposure for 1 day (*p < 0.05).

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  Figure 3. Effect of genistein on the activity of eNOS. Genistein activated eNOS after 10 min (A) and 1 day exposures (B). Lane 1, 5 for control, lane 2, 6 for 0.1 μ m, lane, 3, 7: for 0.1 μ m + L-NAME, lane 4, 8 for 1 μ m.

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  Figure 4. Effect of genistein on the survival of trabecular meshwork cells after exposure for 1 days (* p < 0.05).

Reference

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