Immune Netw.  2012 Oct;12(5):181-188. 10.4110/in.2012.12.5.181.

Immunomodulatory Effects of Dioscoreae Rhizome Against Inflammation through Suppressed Production of Cytokines Via Inhibition of the NF-kappaB Pathway

Affiliations
  • 1College of Pharmacy, Sahmyook University, Seoul 139-742, Korea. kimkj@syu.ac.kr
  • 2College of Pharmacy, Chungbuk University, Cheongju 361-763, Korea.

Abstract

Dioscoreae Rhizome (DR) has been used in traditional medicine to treat numerous diseases and is reported to have anti-diabetes and anti-tumor activities. To identify a bioactive traditional medicine with anti-inflammatory activity of a water extract of DR (EDR), we determined the mRNA and protein levels of proinflammatory cytokines in macrophages through RT-PCR and western blot analysis and performed a FACS analysis for measuring surface molecules. EDR dose-dependently decreased the production of NO and pro-inflammatory cytokines such as IL-1beta, IL-6, TNF-alpha, and PGE2, as well as mRNA levels of iNOS, COX-2, and pro-inflammatory cytokines, as determined by western blot and RT-PCR analysis, respectively. The expression of co-stimulatory molecules such as B7-1 and B7-2 was also reduced by EDR. Furthermore, activation of the nuclear transcription factor, NF-kappaB, but not that of IL-4 and IL-10, in macrophages was inhibited by EDR. These results show that EDR decreased pro-inflammatory cytokines via inhibition of NF-kappaB-dependent inflammatory protein level, suggesting that EDR could be a useful immunomodulatory agent for treating immunological diseases.

Keyword

Dioscoreae Rhizoma; Anti-inflammatory; Inflammatory cytokines; Immunomodulatory agent

MeSH Terms

Blotting, Western
Cytokines
Dinoprostone
Dioscorea
Immune System Diseases
Inflammation
Interleukin-10
Interleukin-4
Interleukin-6
Macrophages
Medicine, Traditional
NF-kappa B
Rhizome
RNA, Messenger
Transcription Factors
Tumor Necrosis Factor-alpha
Water
Cytokines
Dinoprostone
Interleukin-10
Interleukin-4
Interleukin-6
NF-kappa B
RNA, Messenger
Transcription Factors
Tumor Necrosis Factor-alpha
Water
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