Nat Prod Sci.  2018 Sep;24(3):148-154. 10.20307/nps.2018.24.3.148.

Acer okamotoanum Inhibit the Hydrogen Peroxide-Induced Oxidative Stress in C6 Glial Cells

  • 1Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Republic of Korea.
  • 2Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Republic of Korea.


Chronic oxidative stress due to the accumulation of reactive oxygen species (ROS) in neuronal cells ultimately leads to neurodegenerative diseases. The use of natural therapies for the prevention of ROS-induced cell damage and for the treatment of neurodegenerative disorders has shown promising results. In this study, we evaluated the neuroprotective effects of the ethyl acetate (EtOAc) fraction of A. Okamotoanum against the hydrogen peroxide (H₂O₂)-induced oxidative stress in C6 glial cells. Results show that cell viability was decreased in cells incubated with H₂O₂, whereas the addition of EtOAc fraction treatments in such cells significantly increased viability. The EtOAc fraction showed the highest inhibitory activity against ROS production and it also decreased the expressions of inflammatory proteins including cyclooxygenase-2, inducible nitric oxide synthase and interleukin-1β. Furthermore, the EtOAc fraction inhibited apoptosis by regulating the protein expressions cleaved caspase -9, -3, poly ADP ribose polymerase, Bax and Bcl-2. Therefore, these results show that the EtOAc fraction of A. Okamotoanum exhibits neuroprotective effects against H₂O₂ induced oxidative damage by regulating the inflammatory reaction and apoptotic pathway.


Acer okamotoanum; C6 glial cell; Inflammation; Apoptosis; Reactive oxygen species

MeSH Terms

Cell Survival
Cyclooxygenase 2
Hydrogen Peroxide
Neurodegenerative Diseases
Neuroprotective Agents
Nitric Oxide Synthase Type II
Oxidative Stress*
Poly(ADP-ribose) Polymerases
Reactive Oxygen Species
Cyclooxygenase 2
Hydrogen Peroxide
Neuroprotective Agents
Nitric Oxide Synthase Type II
Poly(ADP-ribose) Polymerases
Reactive Oxygen Species
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