Tissue Eng Regen Med.
2017 Apr;14(2):133-141. 10.1007/s13770-017-0030-9.
Effects of Remifentanil Preconditioning Attenuating Oxidative Stress in Human Dermal Fibroblast
- Affiliations
-
- 1Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Mulgeum-eup, Yangsan-si, Gyeongnam 50612, Korea.
- 2Department of Oral Anatomy, School of Dentistry, Pusan National University, Mulgeum-eup, Yangsan-si, Gyeongnam 50612, Korea.
- 3Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University, Mulgeum-eup, Yangsan-si, Gyeongnam 50612, Korea. jiuk@pusan.ac.kr
- KMID: 2377343
- DOI: http://doi.org/10.1007/s13770-017-0030-9
Abstract
- Human dermal fibroblast is essential in wound healing of the skin through the synthesis of extracellular matrix proteins. With respect to oxidative stress, the effects of remifentanil on human dermal fibroblast have received little attention. Therefore, we investigated the effects of remifentanil on the apoptosis and autophagic reaction of human dermal fibroblasts under oxidative stress. The subjects were divided into the following groups: Control group: cells were incubated at 37℃ in a humidified atmosphere with 5% CO₂. Hydrogen peroxide (H₂O₂) group: cells were exposed to H₂O₂ for 2 h. RPC/H₂O₂ group: cells were pretreated with remifentanil for 2 h and exposed H₂O₂ for 2 h. 3-MA/RPC/H₂O₂ group: cells were pretreated with 3-methyladenine (3-MA) and remifentanil for 1 h and 2 h, respectively. We measured cell viability using MTT assay. Western blot analysis was used to determine the expression levels of proteins associated with apoptosis and autophagy. Quantification of apoptotic cells was performed using flow cytometer analysis, and autophagic vacuoles were observed under a fluorescence microscope. Remifentanil treatment increased the proliferation of human dermal fibroblast and decreased apoptotic cell death, enhancing autophagic activity under oxidative stress. However, 3-MA, the autophagy pathway inhibitor, inhibited the protective effect of remifentanil in oxidative stress. This study demonstrates that remifentanil activated autophagy and decreased apoptotic death of human dermal fibroblasts under oxidative stress. Our results suggest that remifentanil may help in the treatment of oxidative stress.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Remifentanil induces autophagy and prevents hydrogen peroxide-induced apoptosis in Cos-7 cells
- Ischemic Preconditioning and the Role of Protein Kinase C in Cultured Retinal Ganglion Cell Line
- Protective effects of remifentanil against Hâ‚‚Oâ‚‚-induced oxidative stress in human osteoblasts
- Effects of propofol-induced autophagy against oxidative stress in human osteoblasts
- Effect of Progesterone on Cultured Human Dermal Fibroblast
