Korean J Physiol Pharmacol.  2017 Mar;21(2):233-239. 10.4196/kjpp.2017.21.2.233.

Hydrogen peroxide attenuates refilling of intracellular calcium store in mouse pancreatic acinar cells

Affiliations
  • 1Department of Physiology, College of Medicine, Konyang University, Daejeon 35365, Korea. hspark@konyang.ac.kr
  • 2Myunggok Medical Research Institute, Konyang University, Daejeon 35365, Korea.

Abstract

Intracellular calcium (Ca²âº) oscillation is an initial event in digestive enzyme secretion of pancreatic acinar cells. Reactive oxygen species are known to be associated with a variety of oxidative stress-induced cellular disorders including pancreatitis. In this study, we investigated the effect of hydrogen peroxide (Hâ‚‚Oâ‚‚) on intracellular Ca²âº accumulation in mouse pancreatic acinar cells. Perfusion of Hâ‚‚Oâ‚‚ at 300 µM resulted in additional elevation of intracellular Ca²âº levels and termination of oscillatory Ca²âº signals induced by carbamylcholine (CCh) in the presence of normal extracellular Ca²âº. Antioxidants, catalase or DTT, completely prevented Hâ‚‚Oâ‚‚-induced additional Ca²âº increase and termination of Ca²âº oscillation. In Ca²âº-free medium, Hâ‚‚Oâ‚‚ still enhanced CCh-induced intracellular Ca²âº levels and thapsigargin (TG) mimicked Hâ‚‚Oâ‚‚-induced cytosolic Ca²âº increase. Furthermore, Hâ‚‚Oâ‚‚-induced elevation of intracellular Ca²âº levels was abolished under sarco/endoplasmic reticulum Ca²âº ATPase-inactivated condition by TG pretreatment with CCh. Hâ‚‚Oâ‚‚ at 300 µM failed to affect store-operated Ca²âº entry or Ca²âº extrusion through plasma membrane. Additionally, ruthenium red, a mitochondrial Ca²âº uniporter blocker, failed to attenuate Hâ‚‚Oâ‚‚-induced intracellular Ca²âº elevation. These results provide evidence that excessive generation of Hâ‚‚Oâ‚‚ in pathological conditions could accumulate intracellular Ca²âº by attenuating refilling of internal Ca²âº stores rather than by inhibiting Ca²âº extrusion to extracellular fluid or enhancing Ca²âº mobilization from extracellular medium in mouse pancreatic acinar cells.

Keyword

Hydrogen peroxide; Intracellular Ca²⁺ stores; Pancreatic acinar cells; Reactive oxygen species; Sarcoplasmic reticulum Ca²⁺ ATPase

MeSH Terms

Acinar Cells*
Animals
Antioxidants
Calcium*
Carbachol
Catalase
Cell Membrane
Cytosol
Extracellular Fluid
Hydrogen Peroxide*
Hydrogen*
Ion Transport
Mice*
Pancreatitis
Perfusion
Reactive Oxygen Species
Reticulum
Ruthenium Red
Thapsigargin
Antioxidants
Calcium
Carbachol
Catalase
Hydrogen
Hydrogen Peroxide
Reactive Oxygen Species
Ruthenium Red
Thapsigargin
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