Mycobiology.  2015 Jun;43(2):131-136. 10.5941/MYCO.2015.43.2.131.

Acetyl Eburicoic Acid from Laetiporus sulphureus var. miniatus Suppresses Inflammation in Murine Macrophage RAW 264.7 Cells

Affiliations
  • 1Laboratory of Veterinary Physiology and Cell Signaling, Cardiovascular Research Institute and College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea. rheemh@knu.ac.kr
  • 2Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea. bsyun@jbnu.ac.kr

Abstract

The basidiomycete Laetiporus sulphureus var. miniatus belongs to the Aphyllophorales, Polyporaceae, and grows on the needleleaf tree. The fruiting bodies of Laetiporus species are known to produce N-methylated tyramine derivatives, polysaccharides, and various lanostane triterpenoids. As part of our ongoing effort to discover biologically active compounds from wood-rotting fungi, an anti-inflammatory triterpene, LSM-H7, has been isolated from the fruiting body of L. sulphureus var. miniatus and identified as acetyl eburicoic acid. LSM-H7 dose-dependently inhibited the NO production in RAW 264.7 cells without any cytotoxicity at the tested concentrations. Furthermore it suppressed the production of proinflammatory cytokines, mainly inducible nitric oxide synthase, cyclooxygenase-2, interleukin (IL)-1beta, IL-6 and tumor necrosis factor alpha, when compared with glyceraldehyde 3-phosphate dehydrogenase. These data suggest that LSM-H7 is a crucial component for the anti-inflammatory activity of L. sulphureus var. miniatus.

Keyword

Acetyl eburicoic acid; Anti-inflammation; Laetiporus sulphureus var. miniatus; Nitric oxide; Proinflammatory cytokine

MeSH Terms

Basidiomycota
Cyclooxygenase 2
Cytokines
Fruit
Fungi
Glyceraldehyde 3-Phosphate
Inflammation*
Interleukin-6
Interleukins
Macrophages*
Nitric Oxide
Nitric Oxide Synthase Type II
Oxidoreductases
Polyporaceae
Polyporales
Polysaccharides
Trees
Tumor Necrosis Factor-alpha
Tyramine
Cyclooxygenase 2
Cytokines
Glyceraldehyde 3-Phosphate
Interleukin-6
Interleukins
Nitric Oxide
Nitric Oxide Synthase Type II
Oxidoreductases
Polysaccharides
Tumor Necrosis Factor-alpha
Tyramine
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