J Korean Biol Nurs Sci.  2015 Nov;17(4):341-347. 10.7586/jkbns.2015.17.4.341.

Anti-Inflammatory Effects of Chrysanthemum indicum Water Extract in RAW 264.7 Cell as a Whole Plant

• Kang K

• KMID: 2173204
• DOI: http://doi.org/10.7586/jkbns.2015.17.4.341

Abstract

PURPOSE
Chrysanthemum indicum (CHI) has been used for edible and medical purposes for a long time in Korea. The purpose of this study was to evaluate the anti-inflammatory effects of CHI water extract in lipopolysaccharides (LPS)-induced RAW 264.7 macrophage cells.
METHODS
To investigate the anti-inflammatory effects on LPS-induced RAW 264.7 macrophage cells, CHI extract as a whole plant was used in this study. RAW 264.7 cells were treated with various concentrations of CHI extract (1, 10, and100 microg/mL). After that Nitric Oxide (NO), inducible nitric oxide synthase (iNOS), interleukin (IL)-1beta, cyclooxygenase (COX)-2 and prostaglandin E2 (PGE2) expression level were measured.
RESULTS
CHI extract significantly suppressed the LPS-induced NO production and decreased the level of iNOS, IL-1beta, COX-2 messenger ribonucleic acid (mRNA) expression and also the down regulation of PGE2 expression in a dose-dependent manner.
CONCLUSION
The present study suggested that CHI extract can be substituted for anti-inflammatory drugs and provide a safe and effective non pharmacological therapeutic approach.

Keyword

Anti-inflammation; Chrysanthemum indicum; RAW 264.7 cell

MeSH Terms

Chrysanthemum*
Dinoprostone
Down-Regulation
Interleukins
Korea
Lipopolysaccharides
Macrophages
Nitric Oxide
Nitric Oxide Synthase Type II
Plants*
Prostaglandin-Endoperoxide Synthases
RNA
Water*
Dinoprostone
Interleukins
Lipopolysaccharides
Nitric Oxide
Nitric Oxide Synthase Type II
Prostaglandin-Endoperoxide Synthases
RNA
Water