Nat Prod Sci.  2016 Sep;22(3):147-153. 10.20307/nps.2016.22.3.147.

Anti-inflammatory Effect of Mangosteen (Garcinia mangostana L.) Peel Extract and its Compounds in LPS-induced RAW264.7 Cells

• Widowati W
• Darsono L
• Suherman J
• Fauziah N
• Maesaroh M
• Erawijantari PP

• KMID: 2355138
• DOI: http://doi.org/10.20307/nps.2016.22.3.147

Abstract

Inflammation plays an important role in host defense against external stimuli such as infection by pathogen, endotoxin or chemical exposure by the production of the inflammatory mediators that produced by macrophage. Anti-inflammatory factor is important to treat the dangers of chronic inflammation associated with chronic disease. This research aims to analyze the anti-inflammatory effects of Garcinia mangostana L. peel extract (GMPE), α-mangostin, and γ-mangostin in LPS-induced murine macrophage cell line (RAW 264.7) by inhibiting the production of inflammatory mediators. The cytotoxic assay of G. mangostana L. extract, α-mangostin, and γ-mangostin were performed by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) to determine the safe and non-toxic concentration in RAW 264.7 for the further assay. The concentration of inflammatory mediators (COX-2, IL-6, and IL-1β) were measured by the ELISA-based assay and NO by the nitrate/nitrite colorimetric assay in treated LPS-induced RAW 264.7 cells. The inhibitory activity was determined by the reducing concentration of inflammatory mediators in treated LPS-induced RAW 264.7 over the untreated cells. This research revealed that GMPE, α-mangostin, and γ-mangostin possess the anti-inflammatory effect by reducing COX-2, IL-6, IL-1β, and NO production in LPS-induces RAW 264.7 cells.

Keyword

Anti-inflammatory; GMPE; α-Mangostin; γ-Mangostin; Macrophages; Inflammatory mediator

MeSH Terms

Cell Line
Chronic Disease
Fibrinogen
Garcinia mangostana*
Inflammation
Interleukin-6
Macrophages
RAW 264.7 Cells
Fibrinogen
Interleukin-6

Figure

• 

  Fig. 1. Chemical structure of (a) α-mangostin and (b) γ-mangostin.

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