Biomol Ther.  2016 Jan;24(1):75-84. 10.4062/biomolther.2015.069.

Rosmarinic Acid Attenuates Cell Damage against UVB Radiation-Induced Oxidative Stress via Enhancing Antioxidant Effects in Human HaCaT Cells

• Fernando P
• Piao MJ
• Kang KA
• Ryu YS
• Hewage SR
• Chae SW
• Hyun JW

• KMID: 2170544
• DOI: http://doi.org/10.4062/biomolther.2015.069

Abstract

This study was designed to investigate the cytoprotective effect of rosmarinic acid (RA) on ultraviolet B (UVB)-induced oxidative stress in HaCaT keratinocytes. RA exerted a significant cytoprotective effect by scavenging intracellular ROS induced by UVB. RA also attenuated UVB-induced oxidative macromolecular damage, including protein carbonyl content, DNA strand breaks, and the level of 8-isoprostane. Furthermore, RA increased the expression and activity of superoxide dismutase, catalase, heme oxygenase-1, and their transcription factor Nrf2, which are decreased by UVB radiation. Collectively, these data indicate that RA can provide substantial cytoprotection against the adverse effects of UVB radiation by modulating cellular antioxidant systems, and has potential to be developed as a medical agent for ROS-induced skin diseases.

Keyword

Antioxidant system; Oxidative stress; Reactive oxygen species; Rosmarinic acid; Ultraviolet B

MeSH Terms

Antioxidants*
Catalase
Cytoprotection
DNA
Heme Oxygenase-1
Humans*
Keratinocytes
Oxidative Stress*
Reactive Oxygen Species
Skin Diseases
Superoxide Dismutase
Transcription Factors
Antioxidants
Catalase
DNA
Heme Oxygenase-1
Reactive Oxygen Species
Superoxide Dismutase
Transcription Factors