Biomol Ther.  2019 Jul;27(4):395-403. 10.4062/biomolther.2018.151.

Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5

• Zhen AX
• Piao MJ
• Hyun YJ
• Kang KA
• Ryu YS
• Cho SJ
• Kang HK
• Koh YS
• Ahn MJ
• Kim TH
• Hyun JW

• KMID: 2455095
• DOI: http://doi.org/10.4062/biomolther.2018.151

Abstract

Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 (PM(2.5)) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and PM(2.5) severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or PM(2.5). Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and PM(2.5).

Keyword

Purpurogallin; Ultraviolet B radiation; Particulate matter 2.5; Oxidative stress; Human HaCaT keratinocytes

MeSH Terms

Antioxidants
Apoptosis Regulatory Proteins
Apoptosis*
DNA
Humans
Keratinocytes*
Oxidative Stress
Particulate Matter*
Phenol
Reactive Oxygen Species
Antioxidants
Apoptosis Regulatory Proteins
DNA
Particulate Matter
Phenol
Reactive Oxygen Species