Biomol Ther.  2015 Jul;23(4):357-366. 10.4062/biomolther.2015.005.

Isorhamnetin Protects Human Keratinocytes against Ultraviolet B-Induced Cell Damage

• Han X
• Piao MJ
• Kim KC
• Madduma Hewage SRK
• Yoo ES
• Koh YS
• Kang HK
• Shin JH
• Park Y
• Yoo SJ
• Chae S
• Hyun JW

• KMID: 2382366
• DOI: http://doi.org/10.4062/biomolther.2015.005

Abstract

Isorhamnetin (3-methylquercetin) is a flavonoid derived from the fruits of certain medicinal plants. This study investigated the photoprotective properties of isorhamnetin against cell damage and apoptosis resulting from excessive ultraviolet (UV) B exposure in human HaCaT keratinocytes. Isorhamnetin eliminated UVB-induced intracellular reactive oxygen species (ROS) and attenuated the oxidative modification of DNA, lipids, and proteins in response to UVB radiation. Moreover, isorhamnetin repressed UVB-facilitated programmed cell death in the keratinocytes, as evidenced by a reduction in apoptotic body formation, and nuclear fragmentation. Additionally, isorhamnetin suppressed the ability of UVB light to trigger mitochondrial dysfunction. Taken together, these results indicate that isorhamnetin has the potential to protect human keratinocytes against UVB-induced cell damage and death.

Keyword

Isorhamnetin; Ultraviolet B; Reactive oxygen species; Human keratinocyte; Programmed cell death

MeSH Terms

Apoptosis
Cell Death
DNA
Fruit
Humans
Keratinocytes*
Plants, Medicinal
Reactive Oxygen Species
DNA
Reactive Oxygen Species