Biomol Ther.  2014 Jul;22(4):301-307.

Fucodiphlorethol G Purified from Ecklonia cava Suppresses Ultraviolet B Radiation-Induced Oxidative Stress and Cellular Damage

• Kim KC
• Piao MJ
• Zheng J
• Yao CW
• Cha JW
• Kumara MH
• Han X
• Kang HK
• Lee NH
• Hyun JW

Abstract

Fucodiphlorethol G (6'-[2,4-dihydroxy-6-(2,4,6-trihydroxyphenoxy)phenoxy]biphenyl-2,2',4,4',6-pentol) is a compound purified from Ecklonia cava, a brown alga that is widely distributed offshore of Jeju Island. This study investigated the protective effects of fucodiphlorethol G against oxidative damage-mediated apoptosis induced by ultraviolet B (UVB) irradiation. Fucodiphlorethol G attenuated the generation of 2, 2-diphenyl-1-picrylhydrazyl radicals and intracellular reactive oxygen species in response to UVB irradiation. Fucodiphlorethol G suppressed the inhibition of human keratinocyte growth by UVB irradiation. Additionally, the wavelength of light absorbed by fucodiphlorethol G was close to the UVB spectrum. Fucodiphlorethol G reduced UVB radiation-induced 8-isoprostane generation and DNA fragmentation in human keratinocytes. Moreover, fucodiphlorethol G reduced UVB radiation-induced loss of mitochondrial membrane potential, generation of apoptotic cells, and active caspase-9 expression. Taken together, fucodiphlorethol G protected human keratinocytes against UVB radiation-induced cell damage and apoptosis by absorbing UVB radiation and scavenging reactive oxygen species.

Keyword

Fucodiphlorethol G; Ultraviolet B; Mitochondria membrane potential; Reactive oxygen species; Human keratinocytes

MeSH Terms

Apoptosis
Caspase 9
DNA Fragmentation
Humans
Keratinocytes
Membrane Potential, Mitochondrial
Oxidative Stress*
Reactive Oxygen Species
Caspase 9
Reactive Oxygen Species