Biomol Ther.  2014 Mar;22(2):136-142.

The Polyphenol Chlorogenic Acid Attenuates UVB-mediated Oxidative Stress in Human HaCaT Keratinocytes

• Cha JW
• Piao MJ
• Kim KC
• Yao CW
• Zheng J
• Kim SM
• Hyun CL
• Ahn YS
• Hyun JW

Abstract

We investigated the protective effects of chlorogenic acid (CGA), a polyphenol compound, on oxidative damage induced by UVB exposure on human HaCaT cells. In a cell-free system, CGA scavenged 1,1-diphenyl-2-picrylhydrazyl radicals, superoxide anions, hydroxyl radicals, and intracellular reactive oxygen species (ROS) generated by hydrogen peroxide and ultraviolet B (UVB). Furthermore, CGA absorbed electromagnetic radiation in the UVB range (280-320 nm). UVB exposure resulted in damage to cellular DNA, as demonstrated in a comet assay; pre-treatment of cells with CGA prior to UVB irradiation prevented DNA damage and increased cell viability. Furthermore, CGA pre-treatment prevented or ameliorated apoptosis-related changes in UVB-exposed cells, including the formation of apoptotic bodies, disruption of mitochondrial membrane potential, and alterations in the levels of the apoptosis-related proteins Bcl-2, Bax, and caspase-3. Our findings suggest that CGA protects cells from oxidative stress induced by UVB radiation.

Keyword

Chlorogenic acid; Human keratinocyte; Ultraviolet B; Oxidative stress; Apoptosis

MeSH Terms

Apoptosis
Caspase 3
Cell Survival
Cell-Free System
Chlorogenic Acid*
Comet Assay
DNA
DNA Damage
Electromagnetic Radiation
Humans
Hydrogen Peroxide
Keratinocytes*
Membrane Potential, Mitochondrial
Oxidative Stress*
Reactive Oxygen Species
Superoxides
Caspase 3
Chlorogenic Acid
DNA
Hydrogen Peroxide
Reactive Oxygen Species
Superoxides