Exp Mol Med.  2013 Jun;45(6):e27.

Wnt5a stimulates chemotactic migration and chemokine production in human neutrophils

Affiliations
  • 1Department of Biological Science, Sungkyunkwan University, Suwon, South Korea. yoesik@skku.edu
  • 2Mitochondria Hub Regulation Center, Dong-A University, Busan, South Korea.
  • 3Department of Hematology-Oncology, Ajou University School of Medicine, Suwon, South Korea.
  • 4School of Nano-Biotechnology and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, South Korea.
  • 5Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University, Seoul, South Korea.

Abstract

Wnt5a is a ligand that activates the noncanonical Wnt signaling pathways (beta-catenin-independent pathways). Human neutrophils expressed several Wnt5a receptors, such as Frizzled 2, 5 and 8. Stimulation of human neutrophils with Wnt5a caused chemotactic migration and the production of two important chemokines, CXCL8 and CCL2. CCL2 production by Wnt5a was mediated by a pertussis toxin-sensitive G-protein-dependent pathway. Wnt5a also stimulated the phosphorylation of three mitogen-activated protein kinases (MAPKs: ERK, p38 MAPK and JNK) and Akt. Inhibition of ERK, p38 MAPK or JNK by specific inhibitors induced a dramatic reduction in Wnt5a-induced CCL2 production. Supernatant collected from lipopolysaccharide-stimulated macrophages induced neutrophil chemotaxis, which was significantly inhibited by anti-Wnt5a antibody. Our results suggested that Wnt5a may contribute to neutrophil recruitment, mediating the inflammation response.

Keyword

chemotaxis; Gi-protein coupled receptor; neutrophils; Wnt5a
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