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Transition of Marker Enzymes of Rat Hepatocyte Organelles in Culture

Song IH, Kim JY, Sung EK, Lee YC

To investigate recovery, growth, and activity of hepatocyte in primary culture after cell separation, the authors followed up the marker enzyme activities of golgi complex, mitochondria and biologic membrane. Thiamine pyrophosphatase,...
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A Comparative Study of Magnetic-Activated Cell Sorting, Cytotoxicity and Preplating for the Purification of Human Myoblasts

Park YG, Moon JH, Kim J

Although cultured myoblast transplantation has been extensively studied as a gene complementation approach to muscular dystrophy treatment, clinical success has still been limited. The inability to adequately isolate and purify...
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Effects of Air Bubble on the Corneal Endothelium

Kim EK, Park JW, Kim HL, Lee YG, Kang SJ, Lim JH

  • KMID: 2336860
  • J Korean Ophthalmol Soc.
  • 1999 Jun;40(6):1480-1486.
During intraocular surgery including cataract, air bubbles occasionally enter the anterior chamber during irrigation. The influences of these air bubbles on the cornea endothelium, however, have yet to be known....
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Cytokine Gene Expression According to Cell Separation of Cord Blood and Bone Marrow

Hong DE, Jeong DC, Han H, Kim HK, Oh CK

  • KMID: 2072482
  • Korean J Pediatr Hematol Oncol.
  • 1999 Apr;6(1):115-123.
PURPOSE: Umbilical cord blood (CB) transplantation is an alternative method instead of allogeneic bone marrow (BM) transplantation. CB transplantation has used grafts with red blood cell (RBC) depletion but...
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Isolation of neural precursor cells from skeletal muscle tissues and their differentiation into neuron-like cells

Park JS, Kim S, Han DK, Lee JY, Ghil SH

  • KMID: 756071
  • Exp Mol Med.
  • 2007 Aug;39(4):483-490.
Skeletal muscle contains several precursor cells that generate muscle, bone, cartilage and blood cells. Although there are reports that skeletal muscle-derived cells can trans-differentiate into neural-lineage cells, methods for isolating...
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Characterization of sphere-forming HCT116 clones by whole RNA sequencing

Chung E, Oh I, Lee KY

PURPOSE: To determine CD133+ cells defined as cancer stem cells (CSCs) in colon cancer, we examined whether CD133+ clones in HCT116 demonstrate known features of CSCs like sphere-forming ability, chemodrug-resistance,...
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Single Cell Dissociation Methods for Flow Cytometric Cell Death Analysis of Hypoxia-Ischemia Injured Newborn Rat Pup Brain

Hwang JH, Sung DK, Choi CW, Kang S, Chang YS, Park WS, Lee M

  • KMID: 2278631
  • Korean J Pediatr.
  • 2005 May;48(5):545-550.
PURPOSE: Newborn brain tissue has to be dissociated into a single cell suspension for flow cytometric analysis of cell death during hypoxia-ischemia. Thus the development of a method to dissociate...
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Oral Pemphigus Vulgaris: A Case Report

Kim IK, Choi JU, Yang JE, Jang JW, Sasikala B, Kim L

  • KMID: 1474297
  • J Korean Assoc Maxillofac Plast Reconstr Surg.
  • 2009 Sep;31(5):414-418.
Pemphigus vulgaris is a chronic autoimmune intraepithelial blistering disease with oral mucosal manifestations that very often precede the skin lesions. The vesicles or bullae are produced by an acantholytic process,...
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Karyotype analysis of cryopreserved mononuclear cells from cord blood

Ku KY, Chu MA, Kim JY, Lee KS

  • KMID: 2184448
  • J Genet Med.
  • 2008 Jun;5(1):55-60.
PURPOSE: The ability to perform chromosome analysis of cryopreserved cord blood mononuclear cells is important for future retrospective studies. We compared the karyotypes of cryopreserved cells with cells before cryopreservation. METHODS:...
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A Comparative Study on Mononuclear Cell Separation and Cryopreservation of Human Cord Blood

Lee KS, Kim KH, Kim MJ, Choi EJ

  • KMID: 2278138
  • Korean J Pediatr Hematol Oncol.
  • 1998 Apr;5(1):148-162.
BACKGROUND: Although the bone marrow transplantation is now recognized as an effective therapy for some hematologic and malignant diseases, HLA-matched related donor is limited. Human umbilical cord blood contains sufficient...
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Characteristics of B Cell proliferation by polysaccharide fraction of Paeonia japonica miyabe

Park HR, Ham YH, Yee ST, Paik SG, Jo SK

BACKGROUND: Paeonia j ap onica Miyabe is a medicinal plant which has been widely used as a component of blood-building decoctions (Chinese medicinal concept : Bu-Xie). The immunopharmacological characteristics of...
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Cell Processing: Current Status and Future Directions

Stroncek D, Read EJ

Specialized clinical cell processing began in the Department of Transfusion Medicine at the National Institutes of Health in 1984. The number and complexity of procedures performed increased quickly and in...
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Detection of fetal erythroid cells from maternal blood using fluorescence in situ hybridization and liquid culture

Han JY, Kim KH, Park JI, Kim IH, Je GH

Fetal nucleated erythrocytes circulating in maternal blood are a potential source of fetal DNA for noninvasive prenatal genetic diagnosis. However, the estimated ratio of fetal to maternal cells is extremely...
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Long-term Cryopreservation of Cord Blood Mononuclear Cells for Hematopoietic Stem Cell Transplantation

Lee KS

  • KMID: 2072468
  • Korean J Pediatr Hematol Oncol.
  • 1997 Apr;4(1):167-180.
BACKGROUND: The use of human umbilical cord blood(CB) as a source of hematopoietic stem cell transplantation for the treatment of pediatric diseases has been well established. In previous study, we...
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Homing-Associated Cell Adhesion Molecules and Cell Cycle Status on the Nucleated Cells in the Bone Marrow, Mobilized Peripheral Blood and Cord Blood

Lee YH, Lee YA, Noh KT, Kim KH, Han JY, Seo SY, Kwon HC, Kim JS, Kim HJ

Homing-associated cell adhesion molecules (H-CAM) on the CD34+ cells play an important role for the engraftment process following hematopoietic stem cell transplantation (HSCT). However, it seems that not only CD34+...
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IL-10 is Predominantly Produced by CD19(low)CD5(+) Regulatory B Cell Subpopulation: Characterisation of CD19 (high) and CD19(low) Subpopulations of CD5(+) B cells

Lee JH, Noh J, Noh G, Choi WS, Lee SS

IL-10 production by CD19(+)CD5(+) B cells was investigated, by determining the expression levels of CD19, a classical B cell marker. Peripheral mononuclear cells were stained with fluorescence-conjugated anti-CD5, anti-CD19, anti-IL-10,...
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A Questionnaire Survey of HLA Crossmatch Tests in Korea (2015)

Yu S, Kang ES, Park MH

BACKGROUND: We carried out a questionnaire survey for laboratories performing human leukocyte antigen-crossmatch (HLA-XM) to provide a basis for laboratory standardization of HLA-XM tests in Korea. METHODS: The questionnaires were distributed...
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Effects of Dimethyl Sulfoxide on the Differentiation of Myocardial and Endothelial Cells

Lee DH, Park YT, Han SS, Lee YC

To elucidate the effects of dimethyl sulfoxide on of myocardial and endothelial cells in culture, the cells were exposed to 10% dimethyl sulfoxide in culture medium for 1 hour at...
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In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood

Park KS, Lee YS, Kang KS

Mesenchymal stem cells (MSCs) have the capabilities for self-renewal and differentiation into cells with the phenotypes of bone, cartilage, neurons and fat cells. These features of MSCs have attracted the...
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Method-Isolation and Culture of Primary Rat Adipose Derived Stem Cells Using Porous Biopolymer Microcarriers

Jin GZ, Park JH, Wall I, Kim HW

Adipose-derived stem cells (ADSCs) are an attractive source of material for mesenchymal stem cell research due to the abundance of adipose and relative ease of access compared with bone marrow....
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