Korean J Phys Anthropol.
2013 Sep;26(3):115-123. 10.11637/kjpa.2013.26.3.115.
The Effects of Phlomis umbrosa Turcz on Osteoclast Differentiation
- Affiliations
-
- 1Department of Anatomy and Institute for Skeletal Disease, School of Medicine, Wonkwang University, Korea.
- 2Plastic & Rconstructive Surgery, School of Medicine, Wonkwang University, Korea.
- 3Department of Obstetrics and Gynecology, School of Medicine, Wonkwang University, Korea.
- 4Division of Anesthesiology and Pain Medicine, Sanbon Medical Center, School of Medicine, Wonkwang University, Korea.
- 5Department of Internal Medicine, Division of Rheumatology and Institute of Wonkwang Medical Science, School of Medicine, Wonkwang University, Korea.
- 6Department of Neurosurgery, Gunsan Medical Center, Wonkwang University, Korea. jang4025@wku.ac.kr
- 7Institute for Environmental Science, School of Medicine, Wonkwang University, Korea.
- KMID: 1488822
- DOI: http://doi.org/10.11637/kjpa.2013.26.3.115
Abstract
- Many bone-related diseases such as osteoporosis, rheumatoid arthritis are occurred by excessive bone resorbing activity of osteoclasts. Recently, many studies have been proceeded to find out the new therapeutic materials from natural products of plants. Phlomis umbrosa Turcz, one of the natural products of plants has been known to improve bone health. However, the precise effects and treatment mechanisms of Phlomis umbrosa Turcz about bone diseases has been unknown. So, we examined the effects of Phlomis umbrosa Turcz on expression of receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) and bone resorption. Also, we investigated the treatment mechanisms of Phlomis umbrosa Turcz relating to osteoclast differentiation. Here, we showed that Phlomis umbrosa Turcz significantly suppressed RANKL-induced osteoclast differentiation and bone resportion. Furthermore, Phlomis umbrosa Turcz suppressed the activation of NF-kappaB in bone marrow macrophage treated RANKL and M-CSF. The mRNA expression of c-Fos, nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP) in BMMs was inhibited by Phlomis umbrosa Turcz. Integrin alphanu, beta3 relating to cell adhesion and dendritic cell-specific transmembrane protein (DC-STAMP) relating to the structure of filamentous actin (F-actin) ring and cathepsin K relating to bone resorbing activity are disrupted too. These results suggest that Phlomis umbrosa Turcz will be a good materials to treat bone diseases like osteoporosis.
Keyword
MeSH Terms
-
Acid Phosphatase
Actins
Arthritis, Rheumatoid
Biological Agents
Bone Diseases
Bone Marrow
Bone Resorption
Cathepsin K
Cell Adhesion
Cytokines
Isoenzymes
Macrophage Colony-Stimulating Factor
Macrophages
NF-kappa B
Osteoclasts
Osteoporosis
Phlomis
RANK Ligand
RNA, Messenger
T-Lymphocytes
Acid Phosphatase
Actins
Biological Agents
Cathepsin K
Cytokines
Isoenzymes
Macrophage Colony-Stimulating Factor
NF-kappa B
RANK Ligand
RNA, Messenger
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Antioxidant Compounds Isolated from the Roots of Phlomis umbrosa Turcz.
- Quantitation and Radical Scavenging Activity Evaluation of Iridoids and Phenylethanoids from the Roots of Phlomis umbrosa (Turcz.) using DPPH Free Radical and DPPH-HPLC Methods, and their Cytotoxicity
- Effect of Sonicated Extract of Treponema Denticola on Osteoclast Differentiation
- Function of Osteoclast-Specific Protein Tyrosine Phosphatase(PTP-oc) on the Osteoclast Differentiation and Anti-resorptive Drug, Bisphosphonates
- Signaling Pathways in Osteoclast Differentiation
