Nat Prod Sci.  2019 Jun;25(2):122-129. 10.20307/nps.2019.25.2.122.

Quantitation and Radical Scavenging Activity Evaluation of Iridoids and Phenylethanoids from the Roots of Phlomis umbrosa (Turcz.) using DPPH Free Radical and DPPH-HPLC Methods, and their Cytotoxicity

• Le DD
• Nguyen DH
• Zhao BT
• Min BS
• Song SW
• Woo MH

• KMID: 2452781
• DOI: http://doi.org/10.20307/nps.2019.25.2.122

Abstract

The roots of Phlomis umbrosa (Turcz.) (Phlomidis Radix) have been traditionally used to treat cold, reduce swelling and staunch bleeding. Four iridoids (1 - 3 and 5) and six phenylethanoid derivatives (4, and 6 - 10) were isolated from the roots of P. umbrosa. A simple, sensitive, and reliable analytical HPLC/PDA method was developed, validated, and applied to determine 10 marker compounds in Phlomidis Radix. Furthermore, the isolates were evaluated for cytotoxic and anti-oxidant activities as well as DPPH-HPLC method. Among them, compounds 4 and 6 - 9 displayed potent anti-oxidant capacities using DPPH assay with IC50 values of 27.7 ± 2.4, 10.2 ± 1.1, 18.0 ± 0.8, 19.1 ± 0.3, and 19.9 ± 0.6 µM, and compounds 6, 8, and 9 displayed significant cytotoxic activity against HL-60 with IC50 values of 35.4 ± 3.1, 18.6 ± 2.0, and 42.9 ± 3.0 µM, respectively.

Keyword

Phlomis umbrosa; antioxidant; developed HPLC/PDA method; cytotoxicity

MeSH Terms

Hemorrhage
Inhibitory Concentration 50
Iridoids*
Methods*
Phlomis*
Iridoids

Figure

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  Fig. 1 The structures of the isolates from the roots of P. umbrosa.

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  Fig. 2 The HPLC chromatograms at wavelength of 247 nm for the standard mixture (A), Phlomidis Radix extract (B), Dipsaci Radix extract (C), and Phlomidis Radix extract without and with DPPH (D), isochlorogenic acid (IS).

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