Exp Mol Med.  1998 Dec;30(4):240-245.

Regulation of fibronectin gene expression by cyclic AMP and phorbol myristate acetate in HT-1080 human fibrosarcoma cells

• Lee BH
• Park RW
• Kim IS

Abstract

We studied the regulation of fibronectin (FN) gene expression by cAMP and phorbol-12-myristate-13-acetate (PMA) in HT-1080 human fibrosarcoma cells. Dibutyryl cAMP increased FN synthesis and mRNA levels, while PMA inhibited the cAMP-induced FN synthesis. In transient transfection assays, cAMP increased FN promoter activity, while PMA paradoxically enhanced the cAMP-induced promoter activity. Stable transfection experiments, however, showed that neither cAMP or PMA alone nor together affected FN promoter activity. These results suggest that PMA antagonizes the cAMP-induced FN gene expression and that both the action of cAMP and the inhibition of its action by PMA may occur at the posttranscriptional level in HT-1080 cells.

Keyword

cAMP; fibronectin; HT-1080; phorbol myristate acetate; protein kinase C

MeSH Terms

Blotting, Northern
Bucladesine/pharmacology*
Bucladesine/antagonists & inhibitors
Enzyme-Linked Immunosorbent Assay
Fibronectins/metabolism
Fibronectins/genetics*
Fibrosarcoma/genetics*
Gene Expression Regulation*
Human
Luciferase/metabolism
Precipitin Tests
Promoter Regions (Genetics)
RNA, Messenger/metabolism
Tetradecanoylphorbol Acetate/pharmacology*
Transfection
Tumor Cells, Cultured
beta-Galactosidase/metabolism