J Korean Med Sci.  2001 Dec;16(6):756-761. 10.3346/jkms.2001.16.6.756.

Hypermethylation of Tumor-related Genes in Genitourinary Cancer Cell Lines

• Chung WB
• Hong SH
• Kim JA
• Sohn YK
• Kim BW
• Kim JW

• KMID: 648358
• DOI: http://doi.org/10.3346/jkms.2001.16.6.756

Abstract

Hypermethylation of CpG island is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed 13 genitourinary cancer cell lines for aberrant DNA methylation of 5 tumor-related genes using methylation- specific polymerase chain reaction (MSP). GSTP1 was methylated in 5 (38.5%), E-cadherin in 1 (8%), VHL in 1 (8%), and MGMT and hMLH1 in none (0%). Six out of thirteen genitourinary cancer cell lines had methylation of at least one of five genes; 5 had one gene methylated, and, 1 had two genes methylated. Methylation of these 5 genes was not detected in any of the bladder cancer cell lines. GSTP1 was methylated in all of the 3 prostate cancer cell lines. We conclude that aberrant hypermethylation may be an important mechanism for the inactivation of cancer-related genes in kidney and prostate cancer cell lines.

Keyword

Urogenital Neoplasms; Genitourinary cancer cell lines; HMLH1; GSTP1; E-cadherin; VHL; MGMT; Hypermethylation; Methylation-specific PCR

MeSH Terms

Bladder Neoplasms/genetics
Cadherins/genetics
*DNA Methylation
DNA Primers
Genetic Screening/methods
Glutathione Transferase/genetics
Human
Isoenzymes/genetics
Kidney Neoplasms/genetics
Ligases/genetics
Male
Neoplasm Proteins/genetics
O(6)-Methylguanine-DNA Methyltransferase/genetics
Polymerase Chain Reaction
Prostatic Neoplasms/genetics
Tumor Cells, Cultured
Urogenital Neoplasms/*genetics