Korean J Vet Res.  2017 Jun;57(2):97-104. 10.14405/kjvr.2017.57.2.97.

Solanum lycopersicum (tomato) ethanol extract elicits anti-inflammatory effects via the nuclear factor kappa B pathway and rescues mice from septic shock

Affiliations
  • 1Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea. rheemh@knu.ac.kr
  • 2College of Korean Medicine, Daegu Hanny University, Gyeongsan 38610, Korea.

Abstract

Solanum lycopersicum, commonly known as tomato, is widely used in raw, cooked, or liquid forms because it contains nutritional compounds that are beneficial for human health, including carotenoids, lycopene, ascorbic acid, vitamins, and minerals. The tomato is perhaps the most widely studied fruit, especially with respect to its cardioprotective effects. In this study, we aimed to identify the anti-inflammatory mechanisms by which the tomato elicits its anti-inflammatory properties. We treated murine macrophage RAW 264.7 cells with a tomato ethanol extract and performed various biochemical assays including nitric oxide inhibition, cell viability, RNA extraction, expression of pro-inflammatory mediators and cytokines, and immunoblotting, as well we assessed cell survival rates. Our results have shown for the first time that a tomato ethanol extract treatment can suppress nitric oxide production in a dose-dependent manner without cytotoxicity. Moreover, it inhibits the expression of pro-inflammatory mediators and cytokines and elicits its anti-inflammatory effects via the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. In addition, administration of tomato syrup potently rescued mice from septic shock induced by lipopolysaccharide injection. Collectively, our results elucidate details regarding the anti-inflammatory mechanisms of tomato.

Keyword

anti-inflammation; cytokines; pro-inflammatory mediators; septic shock; tomato ethanol extract

MeSH Terms

Animals
Ascorbic Acid
B-Lymphocytes
Carotenoids
Cell Survival
Cytokines
Ethanol*
Fruit
Humans
Immunoblotting
Lycopersicon esculentum*
Macrophages
Mice*
Minerals
Miners
NF-kappa B*
Nitric Oxide
Protein Kinases
RAW 264.7 Cells
RNA
Shock, Septic*
Solanum*
Vitamins
Ascorbic Acid
Carotenoids
Cytokines
Ethanol
Minerals
NF-kappa B
Nitric Oxide
Protein Kinases
RNA
Vitamins
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