J Korean Neurol Assoc.  2002 Jul;20(4):385-393.

Genomic Changes in the Striatum of Unilateral 6-hydroxydopamine Lesioned Parkinson Rat Model

• Kim BJ
• Park KW
• Kim H
• Lee DH

Abstract

BACKGROUND: Parkinson's disease is the primary degenerative disease characterized by rigidity, bradykinesia and resting tremor. Even though dopaminergic cell loss of the substantia nigra compacta is a main pathogenesis of the Parkinson's disease, the striatal dysfuntion is major pathophysiology of pakinsonian symptoms.
METHODS
Since gene expression profile can explain the symptomatic varieties of Parkinson's disease, gene expression was investigated in the striatum of rat brain after lesioning of unilateral substantia nigra compacta with 6-hydroxydopamine using cDNA microarray technique for the first time. The expression patterns of 5,200 rat brain cDNAs were screened and clustered according to the function of gene. The expression patterns of enkephaline and substance-P mRNA were also studied for validation of animal preparation.
RESULTS
Various genes involved in apoptosis, cytokines. cytoskeletal molecules, neurotrophic factors, receptors, intracellular Ca2+ metabolism, signal transduction, stress protein, cell cycle regulator protein, and expressed sequence tags(EST) have shown significant expression changes.
CONCLUSIONS
These gene expressions provide the global assessment of the processes involved in secondary change of striatum afforded by dopaminergic denervation at molecular levels.

Keyword

Parkinson's disease; Microarray; 6-hydroxydopamine; Striatum

MeSH Terms

Animals
Apoptosis
Brain
Cell Cycle
Cytokines
Denervation
DNA, Complementary
Enkephalins
Gene Expression
Hypokinesia
Metabolism
Models, Animal*
Nerve Growth Factors
Oligonucleotide Array Sequence Analysis
Oxidopamine*
Parkinson Disease
Rats*
RNA, Messenger
Signal Transduction
Substantia Nigra
Transcriptome
Tremor
Cytokines
DNA, Complementary
Enkephalins
Nerve Growth Factors
Oxidopamine
RNA, Messenger