Yeungnam Univ J Med.  2007 Jun;24(1):24-40.

Identification of Interleukin 1-Responsive Genes in Human Chondrosarcoma SW1354 cells by cDNA Microarray Technology

• Jeon JH
• Jung YW
• Yun DY
• Kim HD
• Kwon CM
• Hong YH
• Kim JR
• Lee CK

Abstract

BACKGROUND: Accumulating evidence shows that interleukin(IL)-1 plays a critical role in inflammation and connective tissue destruction observed in both osteoarthritis and rheumatoid arthritis. IL-1 induces gene expression related to cytokines, chemokines and matrix metalloproteinases by activation of many different transcription factors.
MATERIALS AND METHODS
The chondrosarcoma cell line, SW1353, is known to be a valuable in vitro system for investigating catabolic gene regulation by IL-1beta in chondrocytic cells. To explore and analyze the changes in gene expression by IL-1 responsible for arthritis, SW1353 was treated with IL-1 for 1, 6 and 24 h and then total RNAs were purified for each time. The changes in gene expression were analyzed with 17k human cDNA microarrays and validated by semi-quantitative RT-PCR.
RESULTS
Greater than a two-fold change was observed in 1,200 genes including metallothioneins, matrix metalloproteinases, extracellular matrix proteins, antioxidant proteins, cytoskeleton proteins, cell cycle regulatory proteins, proteins for cell growth and apoptosis, signaling proteins and transcription factors. These changes appeared to be correlate with the pathophysiological changes observed in early osteoarthritis.
CONCLUSION
cDNA microarray analysis revealed a marked variability in gene expression, and provided insight into the overall molecular changes. The result of this study provide initial information for further studies to identify therapeutic targets in osteoarthritis pathogenesis.

Keyword

Interleukin-1beta; Chondrosarcoma; Microarray Analysis; Osteoarthritis

MeSH Terms

Apoptosis
Arthritis
Arthritis, Rheumatoid
Cell Cycle Proteins
Cell Line
Chemokines
Chondrosarcoma*
Connective Tissue
Cytokines
Cytoskeleton
DNA, Complementary*
Extracellular Matrix Proteins
Gene Expression
Humans*
Inflammation
Interleukin-1
Interleukin-1beta
Interleukins*
Matrix Metalloproteinases
Metallothionein
Microarray Analysis
Oligonucleotide Array Sequence Analysis*
Osteoarthritis
RNA
Transcription Factors
Cell Cycle Proteins
Chemokines
Cytokines
DNA, Complementary
Extracellular Matrix Proteins
Interleukin-1
Interleukin-1beta
Interleukins
Matrix Metalloproteinases
Metallothionein
RNA
Transcription Factors