J Korean Soc Microbiol.  2000 Aug;35(4):299-308.

Analysis of X irradiation related genes in HL60 cells using cDNA microarray

• Park KU
• Hwang MS
• Suh SI
• Suh MH
• Kwon TK
• Park JW
• Cho JW
• Choi EJ
• Baek WK

Abstract

Recently developed cDNA microarray or DNA chip technology allows expression monitoring of expression of hundreds and thousands of genes simultaneously and provides a format for identifying genes as well as changes in their activity. In order to search for changes in gene expression after X irradiation in HL60 cells, cDNA microarray technique was done. In this study, expression of 588 human genes (including oncogenes, tumor suppressor genes, cell cycle regulator genes, intracellular signal transduction modulator genes, apoptosis related genes, transcription factor genes, growth factors and receptor genes, cytokine genes, etc) were analyzed. For cDNA microarray analysis mRNAs were extracted from control and 8 Gy-irradiated HL60 cells. As a result the changes in expression of several genes were observed. This alteration of gene expression was confirmed by reverse transcription-polymerase chain reaction. The expression of heat shock 60 KD protein, c-jun, erythroid differentiation factor, CPP32, myeloid cell nuclear differentiation antigen, MAP kinase-activated protein kinase, interleukin-8, monocyte chemotactic peptide 1 and RANTES genes was increased, but the expression of p55CDC gene was decreased after X irradiation.

MeSH Terms

Apoptosis
Cell Cycle
Chemokine CCL5
DNA, Complementary*
Gene Expression
Genes, Regulator
Genes, Tumor Suppressor
HL-60 Cells*
Hot Temperature
Humans
Intercellular Signaling Peptides and Proteins
Interleukin-8
Monocytes
Myeloid Cells
Oligonucleotide Array Sequence Analysis*
Oncogenes
Protein Kinases
RNA, Messenger
Shock
Signal Transduction
Transcription Factors
Chemokine CCL5
DNA, Complementary
Intercellular Signaling Peptides and Proteins
Interleukin-8
Protein Kinases
RNA, Messenger
Transcription Factors