J Korean Acad Conserv Dent.  2009 Sep;34(5):430-441. 10.5395/JKACD.2009.34.5.430.

The comparison of gene expression from human dental pulp cells and periodontal ligament cells

• So H
• Park SH
• Choi GW

• KMID: 1986606
• DOI: http://doi.org/10.5395/JKACD.2009.34.5.430

Abstract

The purpose of this study was to characterize functional distinction between human dental pulp cells(PC) and periodontal ligament cells(PDLC) using cDNA microarray assay and to confirm the results of the microarray assay using RT-PCR. 3 genes out of 51 genes which were found to be more expressed(>2 fold) in PC were selected, and 3 genes out of 19 genes which were found to be more expressed(>2 fold) in PDLC were selected for RT-PCR as well. According to this study, the results were as follows: 1. From the microarray assay, 51 genes were more expressed (2 fold) from PC than PDLC. 2. RT-PCR confirmed that ITGA4 and TGF beta2 were more expressed in PC than in PDLC 3. From the microarray assay, 19 genes were more expressed (2 fold) from PDLC than PC. 4. RT-PCR confirmed that LUM, WISP1, and MMP1 were more expressed in PDLC than in PC. From the present study, different expression of the genes between the PC and PDLC were characterized to show the genes which play an important role in dentinogenesis were more expressed from PC than PDLC, while the genes which were related with collagen synthesis were more expressed from PDLC than PC.

Keyword

cDNA microarray assay; human dental pulp cells; human periodontal ligament cells; RT-PCR; dentinogenesis; collagen synthesis

MeSH Terms

Collagen
Dental Pulp
Dentinogenesis
Gene Expression
Humans
Oligonucleotide Array Sequence Analysis
Periodontal Ligament
Collagen

Figure

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  Figure 1 Overview of DNA microarray methology

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  Figure 2 Microarray Scanning Image

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  Figure 3 M-A plot The log ratios(M=log2R/G) are plotted on the y-axis against the log of the geometric mean of the signal intensities (A=log2R×G/2). M, expression ratio; A, signal intensity; R, Red for Cy5; G, Green for Cy3.

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  Figure 4 Confirmation of differentially expressed genes observed in microarray results. Three genes (ITGA, NEFH, TGF β2) selected from the genes express more than two fold in pulp cells than periodontal ligament cells were analysed by RT-PCR.

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  Figure 5 Confirmation of differentially expressed genes observed in microarray results. Three genes (MMP1, WISP, LUM) selected from the genes express more than two fold in periodontal ligament cells than pulp cells were analysed by RT-PCR.

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