J Korean Acad Pediatr Dent.  2018 May;45(2):242-249. 10.5933/JKAPD.2018.45.2.242.

Comparison of Gene Expression from Supernumerary Dental Pulp and Periodontal Ligament Stem Cells

• Lee S
• Kim J
• Kim J

• KMID: 2430483
• DOI: http://doi.org/10.5933/JKAPD.2018.45.2.242

Abstract

The purpose of this study is to compare the properties of dental pulp and periodontal ligament stem cells from extracted supernumerary teeth by quantitative real-time PCR. Impacted supernumerary teeth in the maxillary anterior region were extracted. Dental pulp and periodontal ligament cells were collected from extracted supernumerary teeth on the same day. After isolation and culture of cells, compare characterization of them by using qRT-PCR. Primer sequences for odontoblasts are ONT, ALP, OCN, DMP-1 and DSPP. On dental pulp group, ONT has the largest quantity of gene expression, followed by OCN, ALP, DMP-1 and DSPP. On periodontal ligament group, ONT has the largest quantity of gene expression, followed by OCN, ALP, DSPP and DMP-1. Analysis of quantitative gene expression data using relative quantification showed that the expression of all genes decreased in periodontal ligament cells. Dental pulp and periodontal ligament stem cells from supernumerary teeth have the properties of odontoblasts. Considering that properties, supernumerary teeth were considered a useful donor site of dental pulp and periodontal ligament stem cells.

Keyword

Supernumerary dental pulp stem cells; Supernumerary periodontal ligament stem cells; Odontoblasts

MeSH Terms

Dental Pulp*
Gene Expression*
Humans
Odontoblasts
Periodontal Ligament*
Real-Time Polymerase Chain Reaction
Stem Cells*
Tissue Donors
Tooth, Supernumerary