J Korean Acad Pediatr Dent.  2019 Aug;46(3):337-342. 10.5933/JKAPD.2019.46.3.337.

Characterization of Human Dental Pulp Cells from Supernumerary Teeth by Using Flow Cytometry Analysis

Affiliations
  • 1Misa Seoul S Dental Clinic, Korea.
  • 2Department of Pediatric Dentistry, School of Dentistry, Dankook University, Korea. jskim@dku.edu

Abstract

The aim of this study was to analyze cells from human dental pulp tissue of impacted supernumerary teeth as stem cells with flow cytometry. Human dental pulp cells from 15 supernumerary teeth were identified their characteristics as stem cells by expression of mesenchymal stem cell markers through flow cytometry analysis at passage 3 and passage 10. Cluster of differentiation (CD) 73, CD 90, CD 34, CD 45 and STRO-1 cell surface markers were used to figure out characteristics of dental pulp stem cells from supernumerary teeth. At passage 3, the cell population showed positive expression of CD 73, CD90 and STRO-1, lacked expression of CD 34 and CD 45. At passage 10, CD 73, CD 90 and STRO-1 showed positive expression while CD 34 and CD 45 showed negative expression. This study indicated that dental pulp stem cells of supernumerary teeth had the properties of mesenchymal stem cells at both early and late passage. Impacted supernumerary teeth could be considered as a noble source of stem cells because of rapid growth and maintaining characteristics of stem cells until late passage.

Keyword

Supernumerary tooth; Dental pulp stem cell; Flow cytometry

MeSH Terms

Dental Pulp*
Flow Cytometry*
Humans*
Mesenchymal Stromal Cells
Stem Cells
Tooth, Supernumerary*
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