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Bioactivity of endodontic biomaterials on dental pulp stem cells through dentin

Javid B, Panahandeh N, Torabzadeh H, Nazarian H, Parhizkar , Asgary S

OBJECTIVES: This study investigated the indirect effect of calcium-enriched mixture (CEM) cement and mineral trioxide aggregate (MTA), as 2 calcium silicate-based hydraulic cements, on human dental pulp stem cells (hDPSCs)...
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Characterization of Human Dental Pulp Cells from Supernumerary Teeth by Using Flow Cytometry Analysis

You Y, Kim J, Shin J, Lee JH, Kim J

The aim of this study was to analyze cells from human dental pulp tissue of impacted supernumerary teeth as stem cells with flow cytometry. Human dental pulp cells from 15...
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Gene Expression of Supernumerary Dental Pulp Related to the Subculture Speed: A Pilot Study

Lee Y, Kim J, Shin J, Kim J

The purpose of this study was to investigate the odontoblast gene expression related to the subculture speed of supernumerary dental pulp stem cells (sDPSCs). The stem cell is undifferentiated cells...
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Treatment of Periodontal Bone Defects with Stem Cells from Inflammatory Dental Pulp Tissues in Miniature Swine

Li Y, Nan X, Zhong TY, Li T, Li A

BACKGROUND: Containing a certain proportion of mesenchymal stem cells, inflammatory dental tissue showed great tissue regeneration potential in recent years. However, whether it is applicable to promote tissue regeneration in...
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Ultrastructure of Substance P Immunopositive Axons in the Human Dental Pulp

Moon BH, Ha JH, Han HM, Kim TH, Park SK, Bae YC

There exists very little information on the ultrastructure of substance P immunopositive (+) fibers in the human dental pulp, which may help in understanding the mechanism for substance P associated...
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Differentiation of CD31-Positive Vascular Endothelial Cells from Organoid Culture of Dental Pulp Stem Cells

Seo EJ, Park JK, Jeong H, Kang JS, Kim HR, Jang IH

The mesenchymal stem cells (MSCs) that reside in dental tissues hold a great potential for future applications in regenerative dentistry. In this study, we used human dental pulp cells, isolated...
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Comparison of Gene Expression from Supernumerary Dental Pulp and Periodontal Ligament Stem Cells

Lee S, Kim J, Kim J

The purpose of this study is to compare the properties of dental pulp and periodontal ligament stem cells from extracted supernumerary teeth by quantitative real-time PCR. Impacted supernumerary teeth in the...
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Distribution of Cold Receptor Transient Receptor Potential Melastatin 8-Immunopositive Axons in the Mouse Dental Pulp and Periodontal Tissue

Kim TH, Lee JS, Kim YS, Bae YC

Transient receptor potential melastatin 8 (TRPM8) plays a crucial role in innocuous cool sensation, acute cold pain and cold-induced hyperalgesia during pathologic conditions. To help understand TRPM8-mediated cold perception in...
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Induction of Prostaglandin Eâ‚‚ by Porphyromonas gingivalis in Human Dental Pulp Cells

Kim SH, Paek YW, Kang IC

Cyclooxygenase-2 (COX-2)-mediated prostaglandin Eâ‚‚ (PGEâ‚‚) plays a key role in development and progression of inflammatory responses and Porphyromonas gingivalis is a common endodontic pathogen. In this study, we investigated induction...
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Melatonin Rescues Human Dental Pulp Cells from Premature Senescence Induced by Hâ‚‚Oâ‚‚

Park S, Bak KJ, Ok CY, Park HJ, Jang HO, Bae MK, Bae SK

Although anti-aging activities of melatonin, a hormone secreted by the pineal gland, have been reported in senescence-accelerated mouse models and several types of cells, its impact and mechanism on the...
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Co-culture of Human Dental Pulp Stem Cells and Endothelial Cells Using Porous Biopolymer Microcarriers: A Feasibility Study for Bone Tissue Engineering

Jin GZ, Kim HW

Delivery of stem cells with osteogenesis while enabling angiogenesis is important for vascularized bone tissue engineering. Here a three-dimensional (3D) co-culture system of dental pulp stem cells (DPSCs) and endothelial...
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Dental Pulp Stem Cells: Current Advances in Isolation, Expansion and Preservation

Rodas-Junco BA, VillicanËœa C

Dental pulp stem cells (DPSCs) are mesenchymal stem cells with high self-renewal potential that have the ability to differentiate into several cell types. Thus, DPSCs have become a promising source...
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Condition medium of cerebrospinal fluid and retinoic acid induces the transdifferentiation of human dental pulp stem cells into neuroglia and neural like cells

Haratizadeh S, Nazm Bojnordi M, Darabi S, Karimi N, Naghikhani M, Ghasemi Hamidabadi H, Seifi M

Cerebrospinal fluid (CSF) contains several molecules which are essential for neurogenesis. Human dental pulp stem cells (hDPSCs) are putatively neural crest cell-derived that can differentiate into neurons and glial cells...
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Efficacy of Human Platelet Rich Fibrin Exudate vs Fetal Bovine Serum on Proliferation and Differentiation of Dental Pulp Stem Cells

Saeed MA, El-Rahman , Helal ME, Zaher AR, Grawish ME

BACKGROUND AND OBJECTIVES: The imperative role of dental pulp stem cells (DPSCs) in regenerative therapy demands an in-vitro expansion which must deal with the safety and ethical problems associated with...
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In vitro characterization of human dental pulp stem cells isolated by three different methods

Jang JH, Lee HW, Cho KM, Shin HW, Kang MK, Park SH, Kim E

OBJECTIVES: In this study, we characterized human dental pulp cells (HDPCs) obtained by different culture methods to establish the most suitable methodology for dental tissue engineering and regenerative endodontic applications. MATERIALS...
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Effect of hypoxia on angiogenesis-related proteins in human dental pulp cells

Kim MK, Kim SJ, Kim Y, Park HJ, Jo MJ, Bae SK, Kim HJ, Bae MK

Dental pulp is a highly vascularized tissue with high regenerative potential. Revascularization of severed vasculature in the tooth is required for pulp healing during avulsed tooth treatment. In this study,...
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Involvement of TRPA1 in the cinnamaldehyde-induced pulpal blood flow change in the feline dental pulp

Kim D, Lee MH, Kim SK

OBJECTIVES: The purpose of this study was to investigate the involvement of TRPA1 in the cinnamaldehyde-induced pulpal blood flow (PBF) change in the feline dental pulp. MATERIALS AND METHODS: Mandibles of...
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The Role of Autonomous Wntless in Odontoblastic Differentiation of Mouse Dental Pulp Cells

Choi H, Kim TH, Ko SO, Cho ES

PURPOSE: Wnt signaling plays an essential role in the dental epithelium and mesenchyme during tooth morphogenesis. Deletion of the Wntless (Wls) gene in odontoblasts appears to reduce canonical Wnt activity,...
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Effect of dentin treatment on proliferation and differentiation of human dental pulp stem cells

Park M, Pang NS, Jung IY

OBJECTIVES: Sodium hypochlorite (NaOCl) is an excellent bactericidal agent, but it is detrimental to stem cell survival, whereas intracanal medicaments such as calcium hydroxide (Ca[OH]2) promote the survival and proliferation...
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Changes in SIRT gene expression during odontoblastic differentiation of human dental pulp cells

Jang YE, Go SH, Lee BN, Chang HS, Hwang IN, Oh WM, Hwang YC

OBJECTIVES: The aim of this study was to investigate the expression of 7 different sirtuin genes (SIRT1-SIRT7) in human dental pulp cells (HDPCs), and to determine the role of SIRTs...
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