Int J Oral Biol.  2016 Sep;41(3):155-161. 10.11620/IJOB.2016.41.3.155.

Effect of hypoxia on angiogenesis-related proteins in human dental pulp cells

• Kim MK
• Kim SJ
• Kim Y
• Park HJ
• Jo MJ
• Bae SK
• Kim HJ
• Bae MK

• KMID: 2354204
• DOI: http://doi.org/10.11620/IJOB.2016.41.3.155

Abstract

Dental pulp is a highly vascularized tissue with high regenerative potential. Revascularization of severed vasculature in the tooth is required for pulp healing during avulsed tooth treatment. In this study, the relative expression of angiogenesis-related proteins was determined in human dental pulp cells using a human angiogenesis proteome profiler array. The proteome profiler array detected differentially expressed angiogenesis-related factors under conditions of hypoxia, which enhances the angiogenic potential of dental pulp cells. We confirmed that hypoxia regulates the mRNA expression of angiogenesis-related factors, including CXCL16 in dental pulp cells. Furthermore, conditioned media of hypoxic pulp cells induced tube-like structures of vascular endothelial cells, which were reduced by the neutralization of CXCL16 function. In conclusion, our data show that angiogenesis-related factors are differentially expressed by hypoxia in dental pulp cells and suggest that CXCL16 may involve in the revascularization of hypoxic dental pulp.

Keyword

Human dental pulp cells; angiogenesis; angiogenic factors; hypoxia; CXCL16

MeSH Terms

Anoxia*
Culture Media, Conditioned
Dental Pulp*
Endothelial Cells
Humans*
Proteome
RNA, Messenger
Tooth
Tooth Avulsion
Culture Media, Conditioned
Proteome
RNA, Messenger