J Korean Acad Conserv Dent.  2005 May;30(3):193-203. 10.5395/JKACD.2005.30.3.193.

Interleukin-8 and MCP(Monocyte Chemoattractant Protein)-1 expression by the Human Dental Pulps in cultures stimulated with Substance P

• Shin HJ
• Park SH
• Choi GW

• KMID: 2045245
• DOI: http://doi.org/10.5395/JKACD.2005.30.3.193

Abstract

The induction of the IL-8 and MCP-1 by the stimulation of Substance P and TNF-alpha (IL-8 agonist) and the specificity for SP using Spantide (SP antagonist) in the dental pulp tissues was measured quantitatively. In addition, the secretion of the IL-8 in the human dental pulp tissue 36 hrs after the stimulation of SP was observed after the stimulation of SP qualitatively. According to this study, the results were as follows: 1. There was the significant IL-8 induction at 36 h after SP (10-4M) stimulation of the pulp tissue comparing with the unstimulated dental pulp tissues (p < 0.05). IL-8 immunostaining was weakly detected along the periphery of the pulp tissue after Mock stimulation and IL-8 immunostaining was detected around the fibroblast in the pulp tissue 36h After SP (10-4M) stimulation, 2. The secretion of MCP-1 from the dental pulp tissues comparing with Mock stimulation was induced at 36 hrs after TNF-alpha (40 ng/ml) stimulation, but no induction with SP(10-4M). TNF-alpha (40 ng/ml) did not induce the IL-8 secretion from the pulp tissue, weak IL-8 immunostaining was detected along the periphery of the pulp tissue. 3. Spantide (10-5M) inhibited IL-8 induction from the pulp tissues 36 h after SP (10-4M) stimulation. These results suggest that SP significantly induces IL-8 recruiting neutrophils in localized human dental pulp tissue. MCP-1 appears to be less involved in the early establishment of pulpal inflammation in response to irritation such as mechanical insult of dentin. SP may have positive relation with the inflammation of the human dental pulp tissues.

Keyword

IL-8; MCP-1; SP; Pulp tissue; Inflammation

MeSH Terms

Dental Pulp*
Dentin
Fibroblasts
Humans*
Inflammation
Interleukin-8*
Neutrophils
Sensitivity and Specificity
Substance P*
Tumor Necrosis Factor-alpha
Interleukin-8
Substance P
Tumor Necrosis Factor-alpha

Figure

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  Figure 1 IL-8 secretion after stimulation of the pulp tissues with Substance P (10-4 M) and TNF-α(40 ng/ml) for 36 and 48 hrs. Data are mean ± SEM of six independent experiments of 1-6 sets of pulp fragments (each set was obtained from one tooth). *Statistically significant, P < 0.05.

• 

  Figure 2 IL-8 secretion after stimulation of the pulp tissues with Substance P (10-4 M) for 36 and 48 hrs. Data are mean ± SEM of six independent experiments of 1-6 sets of pulp fragments(each set was obtained from one tooth).

• 

  Figure 3 IL-8 secretion after stimulation of the pulp tissues with Substance P (10-4 M) and Spantide (10-5 M) for 36 hrs. Data are mean ± SEM of six independent experiments of 1-6 sets of pulp fragments (each set was obtained from one tooth).

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  Figure 4 IL-8 secretion after stimulation of the pulp tissues with TNF-α(40 ng/ml) in the course of time. Data are mean ± SEM of six independent experiments of 6 pulp fragments.

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  Figure 5 MCP-1 secretion after stimulation of the pulp tissues with Substance P (10-4 M) and TNF-α(40 ng/ml) for 36 and 48 hrs. Data are mean ± SEM of six independent experiments of 1-6 sets of pulp fragments(each set was obtained from one tooth).

• 

  Figure 6 MCP-1 secretion after stimulation of the pulp tissues with TNF-α(40 ng/ml) in the course of time. Data are mean ± SEM of six independent experiments of 6 pulp fragments.

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  Figure 7 Expression of IL-8 in the pulp tissue after mock stimulation. (Original magnification, × 200) A. Frozen sections were incubated with IL-8 antiserum. B. Frozen sections were incubated with nonimmunized normal goat serum.

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  Figure 8 Expression of IL-8 in the pulp tissue after SP (10-4M) stimulation. (Original magnification, × 200) A. Frozen sections were incubated with IL-8 antiserum. B. Frozen sections were incubated with nonimmunized normal goat serum.

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  Figure 9 Expression of IL-8 in the pulp tissue after TNF (40 ng/ml) stimulation. (Original magnification, × 200) A. Frozen sections were incubated with IL-8 antiserum. B. Frozen sections were incubated with nonimmunized normal goat serum.

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