Kosin Med J.  2015 Jun;30(1):41-49. 10.7180/kmj.2015.30.1.41.

Effects of L-ascorbic acid on the production of pro-inflammatory and anti-inflammatory cytokines in C57BL/6 mouse splenocytes

• Kong EH
• Ma SY
• Jeong JY
• Kim KH

• KMID: 2308536
• DOI: http://doi.org/10.7180/kmj.2015.30.1.41

Abstract

OBJECTIVE
The imbalance between pro-inflammatory and anti-inflammatory cytokines may underlie different pain states. Although ascorbic acid is the most important physiological antioxidant that affects host defense mechanisms and immune homeostasis, there is limited information on the effects of ascorbic acid on the production of cytokines.
METHODS
In this study, we investigated the in vitro effect of L-ascorbic acid (AA) on the production of pro-inflammatory and anti-inflammatory cytokines by stimulating C57BL/6 mouse splenocytes with the polyclonal activators lipopolysaccharide or concanavalin A.
RESULTS
AA significantly downregulated the expression of IL-6, IL-12, and TNF-alpha at 48 h and 72 h in mouse splenocytes treated with a combination of polyclonal activators and AA. AA treatment also resulted in upregulation of IL-4 and IL-10 at 72 h. These findings demonstrated that AA significantly potentiated production of anti-inflammatory cytokines whereas there was an inverse association between AA and expression of pro-inflammatory cytokines in mouse splenocytes.
CONCLUSION
AA may have potential applications in the reduction of inflammatory pain because of its function in modulating the production of cytokines. However, further in vivo investigations are necessary to elucidate the mechanisms involved.

Keyword

Ascorbic acid; Interleukin; Tumor necrosis factor

MeSH Terms

Animals
Ascorbic Acid*
Concanavalin A
Cytokines*
Defense Mechanisms
Homeostasis
Interleukin-10
Interleukin-12
Interleukin-4
Interleukin-6
Interleukins
Mice*
Tumor Necrosis Factor-alpha
Up-Regulation
Ascorbic Acid
Concanavalin A
Cytokines
Interleukin-10
Interleukin-12
Interleukin-4
Interleukin-6
Interleukins
Tumor Necrosis Factor-alpha