Korean J Psychopharmacol.  2004 Dec;15(4):449-456.

Fluoxetine-induced Changes on Activity of Tryptophan Hydroxylase at RBL-2H3 Cells

• Baik SY
• Jung KH
• Choi MR
• Yang BH
• Kim SH
• Choi IG
• Chai YG

Abstract

OBJECTIVES
The aim of the present study is to explore the effect of fluoxetine on transcription, translation and activity of tryptophan hydroxylase (TPH), and intracellular level of serotonin. METHODS: The expression level of the TPH mRNA and the protein, the TPH enzyme activity, and the intracellular level of serotonin were explored at the fluoxetine-treated RBL-2H3 cells. Real-time RT-PCR and immunoblotting analysis confirmed changes in the expression of TPH mRNA and protein. The activity of TPH was measured using [3H]tryptophan. The intracellular level of serotonin was measured by HPLC. RESULTS: The TPH activity was gradually increased on time from 24hr to 72hr. The real-time RT-PCR also revealed that the TPH mRNA was increased at 12, 24 and 72hr in the fluoxetine-treated RBL-2H3 cells. The immunoblotting analysis also revealed that the TPH protein was decreased at 72hr in the fluoxetine-treated RBL-2H3 cells. The intracellular level of serotonin was increased at 48hr after treatment of fluoxetine. CONCLUSION: Fluoxetine induced the increases of the TPH mRNA, the TPH enzyme activity and intracellular level of serotonin, and the decrease of the TPH protein expression at the RBL- 2H3 cells.

Keyword

Antidepressant; Selective serotonin reuptake inhibitor; Fluoxetine; Tryptophan hydroxylase; Serotonin; RBL-2H3 cell

MeSH Terms

Chromatography, High Pressure Liquid
Fluoxetine
Immunoblotting
RNA, Messenger
Serotonin
Tryptophan Hydroxylase*
Tryptophan*
Fluoxetine
RNA, Messenger
Serotonin
Tryptophan
Tryptophan Hydroxylase