Korean J Physiol Pharmacol.  2011 Oct;15(5):313-317. 10.4196/kjpp.2011.15.5.313.

Intracellular Ca2+ Mobilization and Beta-hexosaminidase Release Are Not Influenced by 60 Hz-electromagnetic Fields (EMF) in RBL 2H3 Cells

• Hwang YH
• Song HS
• Kim HR
• Ko MS
• Jeong JM
• Kim YH
• Ryu JS
• Sohn UD
• Gimm YM
• Myung SH
• Sim SS

• KMID: 2071735
• DOI: http://doi.org/10.4196/kjpp.2011.15.5.313

Abstract

The effects of extremely low frequency electromagnetic fields (EMF) on intracellular Ca2+ mobilization and cellular function in RBL 2H3 cells were investigated. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not produce any cytotoxic effects in RBL 2H3 cells. Melittin, ionomycin and thapsigargin each dose-dependently increased the intracellular Ca2+ concentration. The increase of intracellular Ca2+ induced by these three agents was not affected by exposure to EMF (60 Hz, 1 mT) for 4 or 16 h in RBL 2H3 cells. To investigate the effect of EMF on exocytosis, we measured beta-hexosaminidase release in RBL 2H3 cells. Basal release of beta-hexosaminidase was 12.3+/-2.3% in RBL 2H3 cells. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not affect the basal or 1 microM melittin-induced beta-hexosaminidase release in RBL 2H3 cells. This study suggests that exposure to EMF (60 Hz, 0.1 or 1 mT), which is the limit of occupational exposure, has no influence on intracellular Ca2+ mobilization and cellular function in RBL 2H3 cells.

Keyword

EMF; Ca2+ mobilization; Exocytosis; Beta-hexosaminidase

MeSH Terms

beta-N-Acetylhexosaminidases
Electromagnetic Fields
Exocytosis
Ionomycin
Melitten
Occupational Exposure
Thapsigargin
Ionomycin
Melitten
Thapsigargin
beta-N-Acetylhexosaminidases