Korean J Physiol Pharmacol.  2010 Dec;14(6):427-433. 10.4196/kjpp.2010.14.6.427.

Effect of Extremely Low Frequency Electromagnetic Fields (EMF) on Phospholipase Activity in the Cultured Cells

• Song HS
• Kim HR
• Ko MS
• Jeong JM
• Kim YH
• Kim MC
• Hwang YH
• Sohn UD
• Gimm YM
• Myung SH
• Sim SS

• KMID: 2071716
• DOI: http://doi.org/10.4196/kjpp.2010.14.6.427

Abstract

This study was conducted to investigate the effects of extremely low frequency electromagnetic fields (EMF) on signal pathway in plasma membrane of cultured cells (RAW 264.7 cells and RBL 2H3 cells), by measuring the activity of phospholipase A2 (PLA2), phospholipase C (PLC) and phospholipase D (PLD). The cells were exposed to the EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h. The basal and 0.5 microM melittin-induced arachidonic acid release was not affected by EMF in both cells. In cell-free PLA2 assay, we failed to observe the change of cPLA2 and sPLA2 activity. Also both PLC and PLD activities did not show any change in the two cell lines exposed to EMF. This study suggests that the exposure condition of EMF (60 Hz, 0.1 or 1 mT) which is 2.4 fold higher than the limit of occupational exposure does not induce phospholipases-associated signal pathway in RAW 264.7 cells and RBL 2H3 cells.

Keyword

EMF; Phospholipase A2; Arachidonic acid; Phospholipase C; Phospholipase D

MeSH Terms

Arachidonic Acid
Cell Line
Cell Membrane
Cells, Cultured
Electromagnetic Fields
Magnets
Occupational Exposure
Phospholipase D
Phospholipases
Phospholipases A2
Pyridoxal
Signal Transduction
Type C Phospholipases
Arachidonic Acid
Phospholipase D
Phospholipases
Phospholipases A2
Pyridoxal
Type C Phospholipases